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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 624 results for: DST;

Showing: 441 - 460 of 624 results

  2. pJW1927

    Plasmid
    #163093
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    mScarlet-I^SEC (Lox511I)^TEV::AID*::3xFLAG
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available Since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163093-plasmid-map-sequence-id-320623

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  16. N-terminal 3X Flag Human CASP9 C287A Mutant

    Plasmid
    #198380
    Purpose
    Expresses Human N-Terminal 3X Flag CASP9 Catalytic Mutant in Mammalian Cells
    Depositor
    Hannah Rabinowich
    Article
    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi
    Insert
    N-Terminal 3X Flag Human Caspase-9 C287A Mutant (CASP9 Human)
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Changed Cysteine 287 to Alanine to eliminate prot…
    Promoter
    CMV
    Available Since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198380-plasmid-map-sequence-id-390258

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  17. pSynSRE-Mut-T-Luc

    Plasmid
    #60490
    Purpose
    Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988
    Depositor
    Timothy Osborne
    Article
    Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.
    Insert
    HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
    Use
    Luciferase
    Tags
    Luciferase reporter
    Expression
    Mammalian
    Mutation
    Four point mutations (G->C or A->C) in the …
    Promoter
    Partial HMG-CoA synthase promoter (-324/-225) fus…
    Available Since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    60490-plasmid-map-sequence-id-332535

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  18. pEAT8-137 M1V

    Plasmid
    #173807
    Purpose
    Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells
    Depositor
    Anne Gershenson
    Article
    Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi
    Insert
    SERPiNA1 (SERPINA1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E1M Otherwise this plasmid encodes the most commo…
    Promoter
    T7
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173807-plasmid-map-sequence-id-341159

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  19. pJW1586

    Plasmid
    #121057
    Purpose
    mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    mKate2-T^SEC^AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, C. elegans codon optimized mKate2, and au…
    Expression
    Worm
    Mutation
    Promoter
    Available Since
    June 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    121057-plasmid-map-sequence-id-235972

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  20. p2R3a-VenusYFP-3AT

    Plasmid
    #71269
    Purpose
    Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    VenusYFP
    Use
    Gateway
    Tags
    4xGly linker and T3A pea Pisum sativum ribulose-1…
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71269-plasmid-map-sequence-id-129809

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Showing: 441 - 460 of 624 results