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pST_pvLEA22mer_mutant_2 (pBS0715)

Plasmid

#185220

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_2. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_17 (pBS0712)

Plasmid

#185219

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_17. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_17

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_16 (pBS0711)

Plasmid

#185218

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_16. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_16

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_pvLEA22mer_mutant_14 (pBS0709)

Plasmid

#185217

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_14. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_14

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_pvLEA22mer_mutant_10 (pBS0705)

Plasmid

#185216

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_10

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_PvLEA4_repeats_k5_15 (pBS0767)

Plasmid

#185248

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_15. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k5_15

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_PvLEA4_repeats_k5_1 (pBS0766)

Plasmid

#185247

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k5_1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k4_14 (pBS0764)

Plasmid

#185246

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k4_14. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k4_14

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_PvLEA4_repeats_k4_1 (pBS0763)

Plasmid

#185245

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k4_1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k4_1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k3_11 (pBS0761)

Plasmid

#185243

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_11. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_11

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF-Frb-v1

Plasmid

#201923

Purpose

E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.

Depositor

Richard Cooley, Ryan Mehl

Article

Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

Inserts

FrbA
FrbB
FrbC
FrbD
FrbE

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 (modified), T7 (modified, moderate), T7 (modif…

Available since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSUPER-hHSF1 shRNA

Plasmid

#118345

Purpose

This plasmid expresses shRNA against hHSF1.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF1 shRNA (HSF1 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MYH11-gRNA1

Plasmid

#132587

Purpose

gRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )

Depositor

James Thomson

Article

Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

Insert

MYH11-gRNA1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Rab35 WT

Plasmid

#47424

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 (RAB35 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B EDD

Plasmid

#37188

Depositor

Charles Watts, Darren Saunders

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD (UBR5 Human)

Use

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Please see Depositor Comments

Promoter

CMV

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B EDD C2768A

Plasmid

#37189

Depositor

Charles Watts, Darren Saunders

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD C2768A (UBR5 Human)

Use

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Changed Cysteine 2768 to Alanine

Promoter

CMV

Available since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

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pSG5 EDD C2768A

Plasmid

#37192

Depositor

Charles Watts, Darren Saunders

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD C2768A (UBR5 Human)

Use

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Changed Cysteine 2768 to Alanine; K503R (please s…

Promoter

SV40

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pSF4 CMV 5TOP intron renilla TRICK CTE polyA

Plasmid

#64544

Purpose

5′ TOP TRICK reporter mRNA

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

12x PP7 stem-loops

Use

Tags

24xMS2 stem loops, 5' TOP (5′ terminal oligo…

Expression

Mammalian

Mutation

Promoter

Tetracycline-inducible promoter and 5′ UTR of hum…

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pDule-3-nitroTyrosine (5B)

Plasmid

#85498

Purpose

Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

Insert

3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Use

Tags

None

Expression

Bacterial

Mutation

Y32H H70C D158S I159A L162R

Promoter

lpp (constitutive)

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDule2-3-nitroTyrosine (5B)

Plasmid

#85499

Purpose

Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

Insert

3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Use

Tags

Expression

Bacterial

Mutation

Y32H H70C D158S I159A L162R

Promoter

lpp (constitutive)

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge