We narrowed to 13,479 results for: cas9 genes

1. pCRISPRx-Sth1Cas9-L5

   Plasmid

   #140993

   Purpose

   Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria

   Depositor

   Wilbert Bitter

   Article

   Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

   Inserts

   Sth1Cas9
   TetR
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified; Integrative in mycobactreia

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR-dCas9

   Plasmid

   #125687

   Purpose

   Gene knockdown for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

   Insert

   streptomyces codon optimized spdCas9, sgRNA casette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   ermE*/tipA

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZ8-T_dCas9

   Plasmid

   #74062

   Purpose

   pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   dcas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptac

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. HBT-pcoCas9

   Plasmid

   #52254

   Purpose

   transient expression of pcoCas9 gene in plant cells

   Depositor

   Jen Sheen

   Article

   Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

   Insert

   pcoCas9
   

   Use

   CRISPR; Plant expression

   Tags

   FLAG and NLS

   Expression

   Mutation

   Promoter

   hybrid constitutive promoter 35SPPDK

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP

   Plasmid

   #198863

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human TERT gene

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   TERT sgRNA (TERT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9-beta

   Plasmid

   #169240

   Purpose

   Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACT2-CAS9c

   Plasmid

   #51055

   Purpose

   Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   Cas9c
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   yeast ADH1 promoter

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCas9-Nours

   Plasmid

   #107733

   Purpose

   Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

   Depositor

   Sheng Yang

   Article

   Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Human optimized

   Promoter

   TEF1

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEVA47_dCas9-GFP_AAN_4

   Plasmid

   #231415

   Purpose

   This AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive promoter. Its sgRNAs X & Y are repressible by sgRNAs A & B, respectively. Its GFP gene is repressible by any of sgRNAs X, Y, and C.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   sgRNA-X
   sgRNA-Y
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-Blast_HP1beta_gRNA

    Plasmid

    #127908

    Purpose

    WT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta gene

    Depositor

    Mark Groudine

    Insert

    gRNA for Human 5' HP1beta
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCas9-Blast

    Plasmid

    #52962

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Lentiviral Prep

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_Cas9–superNLS

    Plasmid

    #232430

    Purpose

    Expression plasmid for the SpCas9 nuclease with superNLS

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

    Insert

    Cas9–superNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9_T2A_mCherry sgAAVS1

    Plasmid

    #192480

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+ heiCas9

    Plasmid

    #186929

    Purpose

    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS

    Depositor

    Joachim Wittbrodt

    Article

    Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:

    Insert

    myc-flexible linker-oNLS-Cas9-oNLS
    

    Use

    CRISPR

    Tags

    myc-flexible linker-optimizedNLS and optimizedNLS

    Expression

    Mammalian

    Mutation

    Promoter

    sp6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH-nCas9-PBE

    Plasmid

    #98163

    Purpose

    Expresses CRISPR base editor in rice

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-nCas9-UGI-NLS
    

    Use

    Rice

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. v5 Cas9-eVLP

    Plasmid

    #228468

    Purpose

    Expresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag(C507V)–Cas9
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    MMLVgag(C507V)

    Promoter

    CMV

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCAS9-VP64_GFP

    Plasmid

    #61422

    Purpose

    Expresses dCAS9-VP64 activator with 2A GFP

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Concentrated Lentiviral Prep

    Insert

    dCAS9(D10A,H840A)-VP64_2A_GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    EF1A

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCas9-GFP

    Plasmid

    #78546

    Purpose

    Fusion protein Cas9+GFP

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Fusion protein with Cas9

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9(BB)-2A-mCherry

    Plasmid

    #197420

    Purpose

    Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9-hHDAC3

    Plasmid

    #98591

    Purpose

    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

    Depositor

    Zhaolan Zhou

    Article

    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

    Insert

    dCAS9-HDAC3 (HDAC3 Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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