We narrowed to 6,177 results for: nls

1. pHR_TRE-->2xNLS_IFP_PGK_PuroR

   Plasmid

   #162238

   Purpose

   Lentiviral vector for the expression of TRE-->2xNLS_IFP_PGK_PuroR in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   NLS IFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-P4:gs:nls:VPR

   Plasmid

   #135983

   Purpose

   Expresses the P4 coiled coil fused to the VPR transcriptional activation domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   P4 coiled-coil fused to the VPR activation domain
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

   Plasmid

   #97313

   Purpose

   Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Lentiviral and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. WT A1-LCD +NLS

   Plasmid

   #178658

   Purpose

   Bacterial expression of N-terminally 6His tagged WT A1-LCD (LCD: low-complexity domain, aa 186-320)

   Depositor

   Tanja Mittag

   Article

   Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

   Insert

   WT A1-LCD +NLS (HNRNPA1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJIT163-2NLSCas9

   Plasmid

   #53064

   Purpose

   Expression of rice codon-optimized Cas9 in plant cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   Cas9
   

   Use

   Plant expression

   Mutation

   Rice codon optimized

   Promoter

   2x35S

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xnls-G-Ca-FLITS

   Plasmid

   #191463

   Purpose

   Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   G-Ca-FLITS
   

   Tags

   3xNLS

   Expression

   Mammalian

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2xNLS-pMJ915v2

   Plasmid

   #88916

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. BB1_23_dCAS9(3xFLAG_SV40NLS)

   Plasmid

   #161478

   Purpose

   BB1 encoding a FLAG tagged dCAS9 with a SV40 NLS

   Depositor

   Diethard Mattanovich, Brigitte Gasser

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Insert

   dCAS9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   FLAG Tag, SV40 NLS

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAi14-GFPNLS-MC

   Plasmid

   #87114

   Purpose

   minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   GFPNLSPpA
   

   Use

   CRISPR

   Promoter

   nEF

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMVMagFRET-1-NLS

    Plasmid

    #50748

    Purpose

    Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-1 with NLS
    

    Expression

    Mammalian

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-NLS-ZapCV2

    Plasmid

    #203664

    Purpose

    Expresses zinc sensor ZapCV2 in the nucleus of mammalian cells

    Depositor

    Amy Palmer

    Article

    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

    Insert

    ZapCV2 Zinc sensor
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3E-nlsGFP no-pA

    Plasmid

    #80815

    Purpose

    nlsGFP without pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    p3E-CMV-nlsGFP no pA
    

    Expression

    Mammalian

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-21a_2xNLS_LbCpf1_protein_expression

    Plasmid

    #114366

    Purpose

    Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

    Insert

    2xNLS_LbCpf1
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

    Plasmid

    #79372

    Purpose

    Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    GCN4-sfGFP-p65-hsf1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p16 nlsGPS1-NR

    Plasmid

    #101082

    Purpose

    GA biosensor expression plasmid

    Depositor

    Alexander Jones, Wolf Frommer

    Article

    Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

    Insert

    nlsGPS1-NR
    

    Tags

    cMyc

    Expression

    Plant

    Mutation

    AtGAI E51K E54R, AtGID1C S114A F115A

    Promoter

    p16 (AT3G60245)

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a_MYC deltaNLS_P2A_Hygro_Barcode

    Plasmid

    #120506

    Purpose

    Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC H84NLS (MYC Human)
    

    Use

    Lentiviral

    Mutation

    Deletion of nuclear localization signal sequence

    Promoter

    EF1a

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMVMagFRET-6-NLS

    Plasmid

    #50757

    Purpose

    Expresses MagFRET-6 in the nucleus of mammalian cells. Weak affinity control FRET sensor for MagFRET-1

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-6 with NLS
    

    Expression

    Mammalian

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. 1xNLS-pMJ915v2

    Plasmid

    #88915

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS-nlstdTomato

    Plasmid

    #132985

    Purpose

    full length of nuclear localized tdTomato in pCS expression vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    tdTomato
    

    Tags

    NLS and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. p3E-2a-6MTnlstdTomato

    Plasmid

    #67699

    Purpose

    3' entry clone with 6xmyc nuclear localized tdTomato

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    tdTomato
    

    Tags

    2A-6xMyc-NLS

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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