We narrowed to 14,883 results for: pET

1. pET21a.MJ1228

   Plasmid

   #11410

   Depositor

   Sung-Hou Kim

   Article

   Kim et al Protein Sci. 1997 Oct . 6(10):2268-70.

   Insert

   eukaryotic translation initiation factor
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pET21a.AQ_1575

   Plasmid

   #11416

   Depositor

   Sung-Hou Kim

   Article

   Shin et al Proc Natl Acad Sci U S A. 2002 Jun 11. 9

   Insert

   hypothetical (aq_1575 A. aeolicus)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pET28aAviHisCD72aCTLD

   Plasmid

   #129065

   Purpose

   generation of biotinylated mouse CD72a CTLD protein using bacteria

   Depositor

   Takeshi Tsubata

   Article

   Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

   Insert

   CD72a (Cd72 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28aHisAviCD72aCTLD

   Plasmid

   #129067

   Purpose

   generation of biotinylated mouse CD72a CTLD protein using bacteria

   Depositor

   Takeshi Tsubata

   Insert

   CD72a (Cd72 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28aHisAviCD72cCTLD

   Plasmid

   #129068

   Purpose

   generation of biotinylated mouse CD72c CTLD protein using bacteria

   Depositor

   Takeshi Tsubata

   Insert

   CD72c (Cd72 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28aHisAvihCD72CTLD

   Plasmid

   #129069

   Purpose

   generation of biotinylated human CD72 CTLD protein using bacteria

   Depositor

   Takeshi Tsubata

   Insert

   CD72 (CD72 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TurboID-His6_pET21a

   Plasmid

   #107177

   Purpose

   expresses His6-tagged TurboID in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET_mNG2(1-10)_32aalinker_mNG2(11)

   Plasmid

   #82611

   Purpose

   Bacterial expression vector for split mNeonGreen2 screening

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   split before mutations

   Promoter

   T7

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET15-UbE2W

   Plasmid

   #15809

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2W (UBE2W Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-iLOVf

    Plasmid

    #63723

    Purpose

    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins

    Depositor

    Brian Smith

    Type

    Empty backbone

    Use

    Tags

    TEE, iLOV, 6xHis, thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETDuet-TFIIF

    Plasmid

    #171082

    Purpose

    Co-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permission

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Inserts

    RAP30 (GTF2F2 Human)
    RAP74 (GTF2F1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET30-T7-sfGFP

    Plasmid

    #180757

    Purpose

    pET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-NT*-HRV3CP

    Plasmid

    #162795

    Purpose

    Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

    Depositor

    Gottfried Otting

    Article

    Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

    Insert

    Human rhinovirus 14 3C protease
    

    Use

    Tags

    NT* solubility tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21a-BirA

    Plasmid

    #20857

    Depositor

    Alice Ting

    Article

    Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    BirA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-SnoopCatcher

    Plasmid

    #72322

    Purpose

    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a-SnoopCatcher
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed G842T and D848G of S. pneumoniae adhesin …

    Promoter

    T7

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a MDHP_YEAST

    Plasmid

    #204233

    Purpose

    Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHP_YEAST (MDH3 Budding Yeast)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    The last 3 C-terminal residues (SKL) are the pero…

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-MSP1D1delltaH5

    Plasmid

    #71714

    Purpose

    Membrane scaffold protein (MSP), helix 5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDB179-HotPETase

    Plasmid

    #218701

    Purpose

    Encodes codon optimized HotPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    HotPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28HIS-hAPE1

    Plasmid

    #70757

    Purpose

    bacterial expression of human APE1

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    APEX1 (APEX1 Human)
    

    Use

    Tags

    6-HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lac operon

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-At-TRL

    Plasmid

    #32242

    Depositor

    Stan Fields, Jay Hesselberth

    Article

    Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

    Insert

    A. thaliana tRNA ligase (RNL Mustard Weed)
    

    Use

    Tags

    6xHistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA Polmyerase

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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