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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,134 results for: ttl

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Showing: 441 - 460 of 1134 results

  2. pYHY3

    Plasmid
    #216109
    Purpose
    aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.
    Depositor
    Shannon Sirk
    Article
    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:
    Insert
    NanoLuc
    Use
    Synthetic Biology; E. coli-bacteroides shuttle ve…
    Tags
    3xFLAG tag and BT3630 signal peptide
    Expression
    Bacterial
    Promoter
    P1TDP-A21
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216109-plasmid-map-sequence-id-434372

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  3. pJYDN3

    Plasmid
    #162456
    Purpose
    Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162456-plasmid-map-sequence-id-321464

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  4. HA-IFITM3-F8-TAG

    Plasmid
    #122475
    Purpose
    expresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon
    Available Since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122475-plasmid-map-sequence-id-237716

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  5. pEVA1

    Plasmid
    #58953
    Purpose
    Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.
    Depositor
    Thomas Fox, Thomas Mason
    Article
    Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.
    Insert
    VAR1u, VAR1 recoded for expression from the nucleus
    Use
    Yeast ecoli shuttle vector
    Tags
    preCox4 mitochondrial matrix targeting signal
    Expression
    Yeast
    Mutation
    Synthetic gene in standard genetic code
    Promoter
    ADH1
    Available Since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pJYDN2p

    Plasmid
    #162455
    Purpose
    Positive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-DnbALFA (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    DnbALFA nanobody and HA and myc
    Promoter
    GAL1
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162455-plasmid-map-sequence-id-321462

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  7. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Expression
    Bacterial
    Promoter
    Pveg
    Available Since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158650-plasmid-map-sequence-id-314145

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  11. AD-003

    Plasmid
    #25502
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    AD-003 (NTMT1 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. FFTTH--GFP-Amotl2

    Plasmid
    #112833
    Purpose
    transient/stable overexpression
    Depositor
    Tomasz Prószyński
    Insert
    Amotl2 (Amotl2 Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112833-plasmid-map-sequence-id-217927

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  18. pEF1-Pls3-sfGFP

    Plasmid
    #205750
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls3 (Phyperspank mutant, IPTG inducible)
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    205750-plasmid-map-sequence-id-478438

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  19. pJYDC2

    Plasmid
    #162459
    Purpose
    Yeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162459-plasmid-map-sequence-id-321477

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Showing: 441 - 460 of 1134 results