19,776 results

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eSpCas9(1.1)_No_FLAG_AAVS1_T2

Plasmid

#79888

Purpose

Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Promoter

CBh

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-T2TP

Plasmid

#114725

Purpose

expression in mammalian cells

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

Tol2 transposase

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pKMD106h - intein-Cas9(C574)

Plasmid

#64191

Purpose

Expresses intein-Cas9(C574) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMEH371 CD63 HA

Plasmid

#168220

Purpose

Mammalian expression of tetraspanin CD63 fused to a C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

CD63 (CD63 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-mScarlet-WPRE

Plasmid

#130994

Purpose

AAV vector to drive the expression of ChRmine-mScarlet under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

mScarlet

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_Blinatumomab_PGK_mCherry

Plasmid

#85437

Purpose

[Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Blinatumomab

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Factor IX donor

Plasmid

#182141

Purpose

Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR

Expression

Mammalian

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-Cas9-Act3.0

Plasmid

#178954

Purpose

It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Insert

zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Actb KI

Plasmid

#131479

Purpose

Endogenous tagging of βactin: N-terminal (amino acid position: D2)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Actb, Actb Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pUDP002

Plasmid

#103872

Purpose

Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Inserts

hph
Spcas9 D147Y P411T

Use

CRISPR

Tags

NLS

Expression

Yeast

Mutation

D147Y P411T

Promoter

Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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mClY-N1

Plasmid

#90457

Purpose

A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

Depositor

Joseph Santos-Sacchi

Article

Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

Insert

mClY-N1

Expression

Mammalian

Mutation

EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-CBE N-terminal

Plasmid

#137175

Purpose

AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE N-terminal; U6-protospacer

Use

AAV

Mutation

Cas9 D10A

Promoter

Cbh

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTRK892

Plasmid

#71803

Purpose

Expression of mutated L. gasseri gusA from strong Ppgm promoter

Depositor

Rodolphe Barrangou, Todd Klaenhammer

Article

Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

Insert

gusA3

Expression

Bacterial

Mutation

D573A

Promoter

Ppgm

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRK-ATF3

Plasmid

#26115

Depositor

Yihong Ye

Article

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

ATF3 (ATF3 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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hCathepsin B

Plasmid

#11249

Purpose

Mammalian expression of human cathepsin B

Depositor

Hyeryun Choe

Article

Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

Insert

cathepsin B (CTSB Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pDA083

Plasmid

#131389

Purpose

eGFP expression

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

eGFP

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-flpE-puro

Plasmid

#20733

Purpose

Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker

Depositor

Rudolf Jaenisch

Article

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

FLPe recombinase

Use

Flp/frt

Expression

Mammalian

Promoter

CAGGS

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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pJC005.em

Plasmid

#182738

Purpose

CRISPR-Cas12a genome editing of E. faecium

Depositor

James Collins

Article

Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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fwYellow chromoprotein

Plasmid

#117841

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, fwYellow

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBS185 CMV-Cre

Plasmid

#11916

Purpose

Mammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoter

Depositor

Brian Sauer

Article

Sauer et al New Biol. 1990 May . 2(5):441-9.

Insert

cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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