We narrowed to 10,927 results for: Kars;

1. pDule2-3-nitroTyrosine (5B)

   Plasmid

   #85499

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32H H70C D158S I159A L162R

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS-CG-WT-Cer-VWF

   Plasmid

   #124827

   Purpose

   Expresses multimeric human VWF-ECFP fusion protein. The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.

   Depositor

   Sriram Neelamegham

   Article

   Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

   Insert

   VWF with cyan fluorescent protein fusion between D'D3 and A1 domains (VWF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. B270 + SPRTN sgSTOP

   Plasmid

   #100718

   Purpose

   B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting SPRTN (cloned using BbsI)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. PPP1CA

   Plasmid

   #155843

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   PPP1CA (PPP1CA Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG -YWAA-pGK-HYG

   Plasmid

   #107381

   Purpose

   Mammalian expression of FLAG-YWAA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   CRBN-Y383A/W385A

   Promoter

   CMV

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA

   Plasmid

   #176538

   Purpose

   Mammalian expression of nuclear localized AwaIscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   AwaIscB
   

   Use

   Tags

   Nucleoplasmin NLS-3xHA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLIK S6K (E389-deltaCT) neo

   Plasmid

   #58516

   Purpose

   Lentiviral expression vector for an inducible HA-tagged rat S6K1-E389-deltaCT

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   Rps6kb1 (Rps6kb1 Rat)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T389E, stop codon at amino acid 402

   Promoter

   Available since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pHS1862

   Plasmid

   #205975

   Purpose

   FLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas5 from JAGBLH010000220, Cas8-HNH
   

   Use

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHS1859

   Plasmid

   #205972

   Purpose

   FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Csy3/Cas7 from JAGBLH010000220, Cas8-HNH
   

   Use

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHS1860

    Plasmid

    #205973

    Purpose

    FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas6/Csy4 from JAGBLH010000220, Cas8-HNH
    

    Use

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. hnRNPA2_LC_nocharge

    Plasmid

    #139118

    Purpose

    express hnRNPA2 LC with no charged residues

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all charged residues are changed to S/Q except fo…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a_MESP1_P2A_Hygro_Barcode

    Plasmid

    #120459

    Purpose

    Barcoded lentiviral vector to express MESP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MESP1 (MESP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. QSOX1-luciferase

    Plasmid

    #113352

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    QSOX1-enhancer (QSOX1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. P-Ef1a-mCherry-T2A-RPL22-1xV5

    Plasmid

    #170324

    Purpose

    Expresses V5 and mCherry tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ESR1-Tol2

    Plasmid

    #113382

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    ESR1-enhancer (ESR1 Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP

    Plasmid

    #162337

    Purpose

    Lentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    S. pyogenes sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6; mU6

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. ATP9A-Tol2

    Plasmid

    #113385

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    ATP9A-enhancer (ATP9A Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ATP9A-luciferase

    Plasmid

    #113354

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    ATP9A-enhancer (ATP9A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1P POLE1_1

    Plasmid

    #160762

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. QSOX1-Tol2

    Plasmid

    #113383

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    QSOX1-enhancer (QSOX1 Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only