We narrowed to 13,642 results for: NTS

1. RnBarr1-mVenus

   Plasmid

   #137790

   Purpose

   Visualization of beta-arrestin1

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat beta-arrestin 1 (Arrb1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP4-myc-H2-Dd

   Plasmid

   #135518

   Purpose

   Mammalian expression of myc-tagged H2-Dd

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   H2-Dd (H2-D1 Mouse)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   C121S

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMP71-mIL15-IL12rb1-sushi-il12rb2

   Plasmid

   #174606

   Purpose

   Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDIRECT_26H

   Plasmid

   #91150

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOD_B2515b

   Plasmid

   #91073

   Purpose

   Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtU6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP

   Plasmid

   #169328

   Purpose

   Expression of HaloTag9 located at the microtubules of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CEP41-HaloTag9 (CEP41 Human)
   

   Use

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Venus-7aa-ActA-pEGFP-C1

   Plasmid

   #177405

   Purpose

   To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.

   Depositor

   David Andrews

   Article

   Osterlund et al Protocol Exchange (2022)

   Insert

   ActA tail anchor (actA Listeria monocytogenes)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. (326) pAAV SV40-ZsGreen U6-gRNA

   Plasmid

   #163020

   Purpose

   Fluorescent reporter for Sa gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Zs Green
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CoV2-N-G204R

   Plasmid

   #177953

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (G204R) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G204R

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

    Plasmid

    #169333

    Purpose

    Expression of HaloTag9 at the mitochondrial surface of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    TOMM20-HaloTag9 (TOMM20 Human)
    

    Use

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMOD_B2615

    Plasmid

    #91078

    Purpose

    Module B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    BsaI ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. α1Ic-HE2-pcDNA3

    Plasmid

    #45808

    Depositor

    Edward Perez-Reyes

    Article

    Murbartian et al FEBS Lett. 2002 Sep 25;528(1-3):272-8.

    Insert

    α1Ic (Cacna1i Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBSFI-myr-akt-K179M

    Plasmid

    #49188

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from K to M at position 179 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    K179M

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJ3 SHP2 CS

    Plasmid

    #8319

    Depositor

    Ben Neel

    Article

    Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C459S, mutation of essential catalytic cysteine

    Promoter

    Available since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMOD_B2301

    Plasmid

    #91067

    Purpose

    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMOD_C2517

    Plasmid

    #91086

    Purpose

    Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU3

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. plc242-GFP-WDR24

    Plasmid

    #87050

    Purpose

    mammalian expression of WDR24

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAFW-Ago1

    Plasmid

    #50553

    Purpose

    Expression of FLAG tagged Ago1 in S2 cell

    Depositor

    Yukihide Tomari

    Article

    Kawamata et al Nat Struct Mol Biol. 2009 Sep;16(9):953-

    Insert

    Argonaute1 (AGO1 Fly)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. HypaCas9

    Plasmid

    #138557

    Purpose

    Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    HypaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CABE-T1.17

    Plasmid

    #193260

    Purpose

    Expression of CABE-T1.17 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T1.17
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHBS838 TDP43 RRM-GFP

    Plasmid

    #98249

    Purpose

    TDP43 RRM-GFP reporter construct

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Cell Rep. 2016 Aug 2;16(5):1228-36. doi:

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    Internal GFP to replace RRM domains

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.2 mir-1-1 reporter hsa-mir-122

    Plasmid

    #46673

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-122 (MIR122 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mCerulean-LaminB1-10

    Plasmid

    #55380

    Purpose

    Localization: Nuclear Envelope, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

24. MPRA_minCMV+GFP

    Plasmid

    #204866

    Purpose

    Vector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.

    Depositor

    Philipp Maass

    Article

    Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

    Insert

    minCMV:GFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ZIKV_Sensor_32B_LacZ

    Plasmid

    #75007

    Purpose

    Toehold switch sensor to detect Zika Virus

    Depositor

    Alexander Green, James Collins

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_sensor_32B_LacZ
    

    Use

    Tags

    LacZ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFAB3833

    Plasmid

    #47839

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 8 and BCD1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    see insert

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pOPS0378

    Plasmid

    #133229

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFAB3737

    Plasmid

    #47838

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 7 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 7 and BCD1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    see insert

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBabe puro H-Ras G12V Y40C

    Plasmid

    #12276

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V Y40C (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The Y40C mutat…

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

30. pROF402

    Plasmid

    #155334

    Purpose

    Binary vector for expression of FLuc controlled by the blue light-repressible system 

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_P35Senhancer(-953 to -51)-(C120)5-PhCMVmin-FLuc-T35S_RB
    

    Use

    Luciferase and Synthetic Biology; Eukaryotic expr…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge