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Addgene

We narrowed to 12,578 results for: LIC

Showing: 11501 - 11550 of 12578 results
  1. ER-GA

    Plasmid
    #209871
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
    Depositor
    Insert
    ddGFP A
    Use
    Tags
    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. Mito-RA

    Plasmid
    #209866
    Purpose
    Dimerization dependent fluorescent protein R anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS
    Depositor
    Insert
    ddRFP A
    Use
    Tags
    Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pBBR1MCS-2

    Plasmid
    #85168
    Purpose
    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.
    Depositor
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive (derepressed P-lac)
    Available since
    Nov. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. pL

    Plasmid
    #196286
    Purpose
    35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)
    Depositor
    Insert
    Full length TSWV L antigenome encoding codon-optimized L (RdRp)
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. myc-BioID2-MCS

    Plasmid
    #74223
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
    Depositor
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Myc and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  6. Mito-GA

    Plasmid
    #209865
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS
    Depositor
    Insert
    ddGFP A
    Use
    Tags
    Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3.1-TRPV1exCellHalo

    Plasmid
    #220307
    Purpose
    Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices
    Depositor
    Insert
    rTRPV1exCellHalo (Trpv1 Rat)
    Use
    Tags
    circularly permutated Halotag (Deo et al., Nat Ch…
    Expression
    Mammalian
    Mutation
    linker included to insert cpHaloTag (
    Promoter
    CMV promoter
    Available since
    July 30, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. pTSin EGFP

    Plasmid
    #127695
    Purpose
    Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence
    Insert
    EGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 3, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
  9. ER-B

    Plasmid
    #209870
    Purpose
    Dimerization dependent fluorescent protein B anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
    Depositor
    Insert
    ddGFP B
    Use
    Tags
    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pLR16-Phes*

    Plasmid
    #98783
    Purpose
    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).
    Depositor
    Insert
    pheS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A309G
    Promoter
    Available since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pUDP004

    Plasmid
    #101165
    Purpose
    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
  12. UAS:mCherry

    Plasmid
    #170994
    Purpose
    UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.
    Depositor
    Insert
    UAS:mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Synthetic 14x UAS:Gal4
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. ER-RA

    Plasmid
    #209872
    Purpose
    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
    Depositor
    Insert
    ddRFP A
    Use
    Tags
    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  14. Perox-RA

    Plasmid
    #209869
    Purpose
    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3
    Depositor
    Insert
    ddRFP A
    Use
    Tags
    Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  15. pJRoC30_VP8H

    Plasmid
    #188062
    Purpose
    PROSS-designed versatile peroxidase 8H
    Depositor
    Insert
    PROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
    Use
    Tags
    S. cerevisiae native α-factor prepro-leader signa…
    Expression
    Yeast
    Mutation
    38 PROSS mutations, described in publication
    Promoter
    Available since
    Aug. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. pcDNA3.1-MBII85-5'SS mut

    Plasmid
    #67631
    Purpose
    MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.
    Depositor
    Insert
    MBII85 snoRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutation in 5'ss
    Promoter
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
  17. pCfB2793

    Plasmid
    #64043
    Purpose
    pCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.
    Insert
    GFP
    Use
    Cre/Lox
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1 promoter and none
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
  18. YIP sHRPa-Aga1P

    Plasmid
    #73151
    Purpose
    Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.
    Depositor
    Insert
    sHRPa-Aga1P
    Use
    Tags
    V5 tag
    Expression
    Yeast
    Mutation
    Promoter
    glyceraldehyde-3-phosphate dehydrogenase GPD prom…
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
  19. Opto-CRAC version 1

    Plasmid
    #101245
    Purpose
    Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling
    Depositor
    Insert
    LOV2 and STIM1 cytosolic fragment
    Use
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
  20. pACEC3GH (Hyg)

    Plasmid
    #110084
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 11501 - 11550 of 12578 results