We narrowed to 8,394 results for: Dos;

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pcDNA3-AMPKsub(TA)-YPet-NES

Plasmid

#84636

Purpose

Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

AMPKsub(TA)-YPet-NES

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHR RPS27A K107/113R-3xFlag_IRES-GFP

Plasmid

#198392

Purpose

RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

ubiquitination site double mutant (K107/113R)

Promoter

EF1A

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-SP110C

Plasmid

#65393

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SP110C

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pHH0103_GNA14_G-alpha

Plasmid

#109914

Purpose

Protein expression and purification of GNA14_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNA14_G-alpha (GNA14 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJH4687

Plasmid

#200783

Purpose

Pnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorin

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ins-22 (ins-22 Nematode)

Tags

pHiuorin

Expression

Worm

Promoter

Pnpr-4

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pETM33_KPNA4_Arm

Plasmid

#178495

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KPNA4_Arm (KPNA4 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pH3R-P2A-mCherry-N1

Plasmid

#84332

Purpose

Co-expresses (untagged) Histamine-3 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H3R-P2A-mCherry (HRH3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSMP-DNMT1_1

Plasmid

#36382

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

DNMT1 (DNMT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-SETDB1_1

Plasmid

#36347

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SETDB1 (SETDB1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pLPCX-Cx43-FS-IRES-GFP

Plasmid

#65438

Purpose

Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

frameshift (FS) mutation 35delA

Promoter

CMV

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His

Plasmid

#83453

Purpose

Full-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

polycystin-1 (Pkd1 Mouse)

Tags

2xMyc, 3xFlag, and 6xHis

Expression

Mammalian

Mutation

S4144D

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pETM33_KEAP1_KELCH

Plasmid

#178496

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KEAP1_KELCH (KEAP1 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pSMP-Bmi1_2

Plasmid

#36353

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Bmi1 (BMI1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pMT2 TIA1 RRM-CFP-FRBx5

Plasmid

#103783

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-CFP-FRBx5

Tags

CFP

Expression

Mammalian

Promoter

SV40

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAT3_G-alpha

Plasmid

#109948

Purpose

Protein expression and purification of GNAT3_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAT3_G-alpha (GNAT3 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cx43-Myc

Plasmid

#60693

Purpose

mammalian expression of Myc-tagged rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pEN_TT JunD-HA

Plasmid

#58498

Purpose

Gateway entry vector for an inducible HA-tagged mouse JunD

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

JunD (Jund Mouse)

Use

Entry vector

Tags

HA

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only
pMGIB-HA-hTCAB1

Plasmid

#167460

Purpose

Retroviral expression of HA-TCAB1 (WDR79) in mammalian cells

Depositor

Steven Artandi

Article

Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

Insert

TCAB1 (WRAP53 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGEXNKI-GST-3C-LIC

Plasmid

#108710

Purpose

Expression of a GST-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

GST-3C protease cleavage site

Expression

Bacterial

Promoter

tac

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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ADGRL3-CTF-Flag

Plasmid

#201219

Purpose

pCDNA3.1 with kozak (GCC), ADGRL3 C-terminal fragment and C-terminal Flag

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

Insert

ADGRL3 (Adgrl3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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