We narrowed to 12,114 results for: LEA

Showing: 4601 - 4620 of 12114 results

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pEPOR0SP0013

Plasmid

#117521

Purpose

Level0 Golden Gate part: CDS, SpCas9-p

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (with stop codon)

Use

CRISPR

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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366 pTol2-CherryDest

Plasmid

#73487

Purpose

promoterless Tol2 vector with mCherry tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signal

Depositor

Nathan Lawson

Publication

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

mCherry-attR1/attR2

Mutation

H30R (please see depositor comments below)

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBT374_pattB-pCA-GFP-pA-attBSwa

Plasmid

#52553

Purpose

for PhiC31 integrase-mediated cassette exchange

Depositor

Liqun Luo

Publication

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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CBS-6266

Plasmid

#253636

Purpose

dBa1Cas12a3 expression

Depositor

Chase Beisel

Publication

Dmytrenko et al Nature. 2026 Jan;649(8099):1312-1321. do

Insert

dBa1Cas12a2 E1032A C-terminal His tag

Tags

His

Expression

Bacterial

Mutation

E1032A

Promoter

T7

Available since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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HER2Nb-C AND (A OR T)-SpyTag

Plasmid

#243790

Purpose

Expresses multifunctional construct enabling the AND(OR)-gated release of a HER2 Nanobody from materials in response to HRV-3C AND (sortase eSrtA(2A9) OR TEV) treatment.

Depositor

Cole DeForest

Publication

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

HER2 nanobody

Tags

6x His tag and SsrA

Expression

Bacterial

Available since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

Plasmid

#226011

Purpose

CasRx guide RNA cloning backbone (lenti)

Depositor

Silvana Konermann

Publication

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Type

Empty backbone

Use

Lentiviral

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pNon-target

Plasmid

#214053

Purpose

Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.

Depositor

Raymond Staals

Publication

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

Non-target sequence

Use

Non-target rna plasmid

Expression

Bacterial

Promoter

trc promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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BsaI-tagRFP-BsaI-pUC-kan

Plasmid

#170771

Purpose

test plasmid for Golden Gate assembly, encodes fluorescent red protein

Depositor

Ichiro Matsumura

Publication

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Insert

tagRFP

Promoter

none

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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MDO2-c004

Plasmid

#173104

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD2 (MACROD2 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

K7-D243

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b

Plasmid

#164862

Purpose

Inducible expression of BzCas13b nuclease in bacteria.

Depositor

Chase Beisel

Publication

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

Bergeyella zoohelcum Cas13b

Expression

Bacterial

Promoter

T7 Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRATIO4214

Plasmid

#141314

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Publication

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mCerulean and mScarlet-I

Expression

Plant

Promoter

UBQ10 promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWY

Plasmid

#112901

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mCitrine.

Depositor

Sebastian Schornack

Publication

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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p5xEGFP-N1

Plasmid

#122165

Purpose

Expression of 5xEGFP

Depositor

Georg Mayr

Publication

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

5xEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p7xEGFP-N1

Plasmid

#122167

Purpose

Expression of 7xEGFP

Depositor

Georg Mayr

Publication

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

7xEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p12xEGFP-N1

Plasmid

#122172

Purpose

Expression of 12xEGFP

Depositor

Georg Mayr

Publication

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

12xEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p3XFlag-YedK

Plasmid

#84567

Purpose

Expresses YedK in mammalian cells

Depositor

Douglas Feldman

Publication

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

YedK

Tags

3xFlag and C-Myc

Expression

Mammalian

Promoter

CMV

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCL0950

Plasmid

#252418

Purpose

KPNB1 tagged on the N-terminus with mCherry.

Depositor

Andrew Bowman

Publication

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

KPNB1 (KPNB1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pET28-SpyCatcher002-MBP

Plasmid

#216295

Purpose

Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells

Depositor

Mark Howarth

Publication

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SpyCatcher002-MBP

Tags

His6 tag and TEV cleavage site

Expression

Bacterial

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMJ235: pRS426-His-MBP-GtFz1_Native

Plasmid

#205254

Purpose

S. cerevisiae expression vector for GtFz1 native guide

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Tags

10xHis, MBP

Expression

Yeast

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO_Ollas.S.V5_mCherry-NLS

Plasmid

#178211

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

Nuclear Localization Signal and Ollas.S.V5

Promoter

eF1a

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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