We narrowed to 13,804 results for: TIM

1. pNB_EA_R1CiM_4EGFP_R3EGFPi2

   Plasmid

   #185968

   Purpose

   Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

   Depositor

   Sangram Bagh

   Article

   Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

   Insert

   Molecular IMPLY gate
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2xNLS-NLP-cMyc AspCas12a

   Plasmid

   #182120

   Purpose

   pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   2xNLS-NLP-cMyc AspCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-ATG13 (1-197)

   Plasmid

   #188074

   Purpose

   To make ATG101/ATG13 HORMA complex for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon-optimized ATG13 (1-197)

   Promoter

   CMV

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-MMETn-del-gRNA2

   Plasmid

   #185007

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   MMETn-del-gRNA2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-IAP-del-gRNA1

   Plasmid

   #185002

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   IAP-del-gRNA1 (Akp3 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-MMERVK9C-del-gRNA1

   Plasmid

   #185004

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   MMERVK9C-del-gRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-MMETn-del-gRNA1

   Plasmid

   #185006

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   MMETn-del-gRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX458-MMERVK9C-del-gRNA2

   Plasmid

   #185005

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   MMERVK9C-del-gRNA2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX458-IAP-del-gRNA2

   Plasmid

   #185003

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   IAP-del-gRNA2 (Akp3 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TOMM20-mTurquoise-RhoA-G14V-deltaCaaX

    Plasmid

    #176123

    Purpose

    Constitutively active, mitochondrial localized RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    RHOA (RHOA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G14V

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEntr1a-SspB-HaloTag-TIAM1-DHPH

    Plasmid

    #176134

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS313_ATP3G919C

    Plasmid

    #120255

    Purpose

    Expression of ATP3-7 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G919 changed to C; Alanine 307 changed to Proline

    Promoter

    ATP3

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS313_ATP3mut

    Plasmid

    #120256

    Purpose

    Expression of ATP3-67 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 304 changed to Asparagine; Alanine 307…

    Promoter

    ATP3

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBI-CMV1-mir144~451-CMV2-mir1

    Plasmid

    #164664

    Purpose

    Express miR-144~451 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144~451 (MIR451A, MIR144 Human)
    miR-1-1 (MIR1-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA™5/FRT/CMV_promoter-ciCas9-E2A-mRFP

    Plasmid

    #164132

    Purpose

    Expresses ciCas9 and mRFP in mammalian cells (an insertion vector to generate ciCas9-knockin cell lines)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    ciCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKM33

    Plasmid

    #154167

    Purpose

    Aedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Rel2 (long isoform)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    C-terminal IκB domain deleted

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. INCTbiosyn-pUB-HspINT4

    Plasmid

    #127513

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 4.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 4 coding sequence codon optimized for H. sapiens expression.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. INCTbiosyn-pUB-HspINT5

    Plasmid

    #127514

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 5.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 5 coding sequence codon optimized for H. sapiens expression.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)

    Plasmid

    #133796

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1 (truncated C-terminal domain only)
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    C-terminal domain only

    Promoter

    CMV and T7

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)

    Plasmid

    #133798

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1(T114A/F115A)
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    T114A/F115A

    Promoter

    CMV and T7

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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