We narrowed to 6,447 results for: nls
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Plasmid#199251PurposeExpression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)DepositorInsertSaCas9.D10A nickase
UseCRISPRTags3XHA epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA
Plasmid#113685PurposeAn EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.DepositorInsertinverted de-catalyzed SaCas9
UseAAV, CRISPR, Cre/Lox, and Synthetic BiologyTagsNLS and VP64Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT (PGK) NanoLuc
Plasmid#208576PurposeEmpty NanoLuc Lentiviral reporterDepositorInsertsNanoLuciferase
d2eGFP
Empty response element
UseLentiviral, Luciferase, and Synthetic BiologyTags3x SV40 NLS and PESTExpressionMammalianPromoterPGK and miniPAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmiCas9-msa
Plasmid#164833PurposemiCas9-msa fusion protein expressing plasmidDepositorInsertmiCas9-msa
UseCRISPRTagsFLAG and NLSExpressionMammalianMutationcontains mSA sequence range is 5106-5447 and Brex…PromoterCMVAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
AALN-BE4max
Plasmid#138161PurposeC-to-T base editorDepositorInsertrAPOBEC1(AALN)-nSpCas9-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 R33A + K34A + H122L + D124N; with…PromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6-hGRIN2B-CAG-ps-SaCas9
Plasmid#102853PurposeA single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editingDepositorInsertsa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B S. aureus, Synthetic, Human)
UseCRISPRTags3X Flag and NLSExpressionMammalianPromoterU6 promoter;Available SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKS7107
Plasmid#89051PurposeExpresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9DepositorInsertsNm crRNA
Nm tracrRNA
hNmCas9
UseCRISPRTagsHA tag, NLS, and SV40 NLSPromoterEF1a and human U6Available SinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-miRFP670nano-PGK-Blast
Plasmid#196722PurposeTet-inducible expression of Cas9-T2A-miRFP670nanoDepositorInsertCas9-T2A-miRFP670nano
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSPromoterTRE, PGKAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEMS1384
Plasmid#29304PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).DepositorInsertPle185
TagsEGFP-NLSAvailable SinceNov. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHS2056
Plasmid#239837PurposeOMEGAOff + omegaRNA scaffold AAV expressionDepositorInsertDNMT3A-DNMT3L-NovaIscB-KRAB::NovaIscB omegaRNA scaffold
UseAAVTagsHA and SV40 NLSMutationInsertion of REC domain from NbaCas9-1 + E137K/E4…PromoterEFS; human U6Available SinceJuly 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1012 - pAAV EF1a DIO Nuc-eYFP
Plasmid#75082PurposeAn AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.DepositorInsertNuc-eYFP
UseAAV and Cre/LoxTagsNLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRETightBI-RY-HMGA2mutant
Plasmid#31470DepositorInserteYFP and mCherry
TagsNLSExpressionMammalianAvailable SinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pvPE-V4-Puro
Plasmid#240290PurposeEncodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene into mammalian cells for gene editing.DepositorInsertpvPE-V4-Puro
UseCRISPRTagsSV40 NLS and c-Myc NLSExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPGK-GZF3-DD
Plasmid#35093DepositorInsertZFN GZF3-Fok(DD)
TagsHA and SV40 NLSExpressionMammalianMutationcontains the "DD" obligate heterodimer …PromoterPGKAvailable SinceMay 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-GFP (PX458)
Plasmid#129026Purposeinactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNADepositorInsertdCas9-hudTET1CD, SgRNA cloning site
TagsHA-Tag, NLS and T2A-EGFPExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-SS-257
Plasmid#68774PurposeC Trans V2DepositorInsertC-terminal Sensor 1 V2
TagsNLS and flagExpressionMammalianAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-zAsCpf1
Plasmid#107185Purposeplasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)DepositorInsertAsCpf1
UseCRISPRTagsNLS-3xHAAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB316
Plasmid#68644PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9 ABE-Umax-ex1
Plasmid#222141PurposeAdenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9
UseCRISPR; Zebrafish expressionTagsBipartite NLSPromoterT3Available SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY008 (pcDNA3.1-hPbCpf1)
Plasmid#69980PurposeExpresses humanized LPbCpf1DepositorInserthPbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only