We narrowed to 11,748 results for: pcDNA

1. pcDNA3.1-TRBP-L1+D1+L2-myc/His

   Plasmid

   #180599

   Purpose

   Expressing various parts of TRBP

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

   Insert

   TRBP (TARBP2 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-MAVS-M1A(KaganF58)

   Plasmid

   #52001

   Purpose

   Expresses the human MAVS CDS containing the point mutation M1A

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Met 1 to Ala

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3 PBD wt (Nigg AH12)

   Plasmid

   #41162

   Depositor

   Erich Nigg

   Article

   Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

   Insert

   PBD (PLK1 Human)
   

   Use

   Tags

   3xMyc and PreScission Site

   Expression

   Mammalian

   Mutation

   Contains Plk1-PBD only (aa 326–603)

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-Flt3-Y591F/Y919F

   Plasmid

   #74497

   Purpose

   Overexpression of FLT3 Y591F/Y919F mutant in mammalian cells

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

   Insert

   FLT3 (FLT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y591F/Y919F, T227M

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1-hRIPK1-Flag-S320A

   Plasmid

   #112491

   Purpose

   Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S320A

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-NBid-PhoCl1-CBid

   Plasmid

   #164050

   Purpose

   Optogenetic manipulation of cell apoptosis using PhoCl1

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   NBid-PhoCl1-CBid
   

   Use

   Tags

   kozak sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promotor

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. WFS1 P724L-Flag-pcDNA3

   Plasmid

   #13012

   Depositor

   Fumihiko Urano

   Article

   Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

   Insert

   Wolfram Syndrome 1 (WFS1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed P 724 to L

   Promoter

   Available since

   Sept. 26, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5/FRT/TO GFP HSPH3

   Plasmid

   #19482

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   HSPH3 (HSPA4L Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA 3.2-FUS-1-373aa-V5

   Plasmid

   #29611

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS-1-373aa (FUS Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Contains FUS aa# 1-373

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-Ha-Brca1 S1423A/S1524A

    Plasmid

    #32539

    Depositor

    Michael Kastan

    Article

    Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S1423A/S1524A

    Promoter

    CMV/rabbit B-Globin

    Available since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

11. FLAG-KLF12/pcDNA3.1-Neo

    Plasmid

    #81069

    Purpose

    Expression of epitope-tagged human KLF12 in mammalian cells.

    Depositor

    Julian Downward

    Article

    Godin-Heymann et al Oncogene. 2016 Jun 23;35(25):3324-34. do

    Insert

    FLAG-KLF12 (KLF12 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. IRE1b(K536A).9E10.pCDNA3

    Plasmid

    #21881

    Depositor

    David Ron

    Insert

    IRE1b (Ern2 Mouse)
    

    Use

    Tags

    Myc (9E10)

    Expression

    Mammalian

    Mutation

    K536A

    Promoter

    Available since

    Nov. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3 c-SRC (250-536)

    Plasmid

    #42208

    Depositor

    Robert Lefkowitz

    Article

    Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

    Insert

    c-SRC (250-536) (SRC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains amino acids 250-536

    Promoter

    CMV

    Available since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5/FRT/TO GFP DNAJB5

    Plasmid

    #19500

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJB5 (DNAJB5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)Gα15-244-NLuc

    Plasmid

    #207154

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNA15 (GNA15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position E(244)N

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP Cav2.2 HA D122A pcDNA3

    Plasmid

    #206120

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cav2.2 HA E1658A (EIVA) pcDNA3

    Plasmid

    #206114

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658A

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cav2.1 EIVA (E1707A) pcDNA3

    Plasmid

    #206125

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1707A

    Promoter

    CMV

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cav2.1 HA EIA (E320A) pcDNA3

    Plasmid

    #206124

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-HA-REDD1 77-232 (#426)

    Plasmid

    #99921

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal deletion; encodes AA 77-232

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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