We narrowed to 7,632 results for: ALP
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Plasmid#196618PurposeExpresses SARS-CoV-2 RBD protein from the BQ.1.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-BQ.1.1
TagsAVI, HRV3C, and scFcExpressionMammalianMutationG339D, R346T, S371F, S373P, S375F, T376A, D405N, …Available SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)
Plasmid#192704PurposeExpression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
TagsIg k-chain leader sequence, SNAPf-tag, and meGFPm…ExpressionMammalianMutationmeGFPm: gluatmic acid 142 to asparagine and histi…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)
Plasmid#192784PurposeExpression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
TagsIg k-chain leader sequence, SNAPf-tag, and mXFPmExpressionMammalianMutationmXFPm: tryptophan 66 to phenylalanine, gluatmic a…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
IL2-AH-2A-Cherry-RhoAQ63L in pmCherryN1
Plasmid#187287PurposeExpress pmCherry-IL2-AH-2A-RhoA Q63LDepositorTagsmCherryExpressionMammalianMutationAH-2A-RhoA Q63LPromoterCMVAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
IL2-AHDC2-2A-CherryRhoAQ63L in pmCherryN1
Plasmid#187292PurposeExpress pmCherry-IL2-AH-deltaC2-2A-RhoA Q63LDepositorTagsmCherryExpressionMammalianMutationAH-deltaC2, RhoA Q63LPromoterCMVAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-SD1-BA.2-AVI
Plasmid#184534PurposeExpresses SARS-CoV-2 RBD-SD1 domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-SD1-BA.2 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationG339D, S371F, S373P, S375F, T376A, D405N, R408S, …Available SinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-BA.2-AVI
Plasmid#184531PurposeExpresses SARS-CoV-2 S2P protein from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-BA.2 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24-, P25-, P26-, A27S, G142D, V213G, G339D…Available SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-SD1-K417N-L452R-T478K-AVI
Plasmid#176457PurposeExpresses SARS-CoV-2 RBD-SD1 domain containing K417N-L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-SD1-K417N-L452R-T478K (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationK417N, L452R, T478KAvailable SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-SD1-K417N-E484K-N501Y-AVI
Plasmid#176450PurposeExpresses SARS-CoV-2 RBD-SD1 domain containing K417N-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-SD1-K417N-E484K-N501Y (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationK417N, E484K, N501YAvailable SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-SD1-K417T-E484K-N501Y-AVI
Plasmid#176451PurposeExpresses SARS-CoV-2 RBD-SD1 domain containing K417T-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-SD1-K417T-E484K-N501Y (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationK417T, E484K, N501YAvailable SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-B.1.617-E154K-L452R-E484Q-D614G-P681R-AVI
Plasmid#176331PurposeExpresses SARS-CoV-2 S2P protein from the B.1.617 variant containing E154K-L452R-E484Q-D614G-P681R mutations with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-B.1.617 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationE154K, L452R, E484Q, D614G, P681RAvailable SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Cstem(7A)-Tac(5A)
Plasmid#162499PurposeExpresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a with mutations is inserted between GFP and Tac(5A).DepositorInsertTagsGFPExpressionMammalianMutationThe five theronine and two serine residues are mu…Available SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX304_zeo_mmFbxw7(res)
Plasmid#160106PurposeExpresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNADepositorAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_AKT1_p.V201I
Plasmid#81453PurposeGateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_AKT1_p.R370C
Plasmid#81416PurposeGateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQ-Elo-HB-C-A(1-772)
Plasmid#171085PurposeCo-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOBDepositorTagsHisExpressionBacterialMutationDeleted N-term 26 amino acids for expression purp…PromoterT5 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Go1-CASE
Plasmid#168123PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at L91/G92 within GNAO1Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCX-TRI-2A
Plasmid#74674Purposemammalian expression of TRI-2A (hHO1/F2A/hCD73/F2A/hCD39)DepositorTagsF2AExpressionMammalianPromoterpCAGGSAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41
Plasmid#213024PurposeExpresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusionDepositorInsertLINE-1 ORF2p (238-1061)
TagsHis6-MBP-3CExpressionBacterialPromoterT7 / LacAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-Volton2-ST-WPRE
Plasmid#172907PurposeAAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequenceDepositorHas ServiceAAV1InsertVoltron2-ST
UseAAV and Cre/LoxTagssoma localization targeting sequenceExpressionMammalianMutationA122DPromoterhSynapsin1Available SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only