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We narrowed to 14,052 results for: CRISPR-Cas9

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Showing: 4661 - 4680 of 14052 results

  4. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Promoter
    Tight TRE promoter
    Available Since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87360-plasmid-map-sequence-id-473508

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  6. pDIV515

    Plasmid
    #177703
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    tRNA-sgRNA-tRNA
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177703-plasmid-map-sequence-id-353317

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  7. pDIV537

    Plasmid
    #177704
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    tRNA-sgRNA-tRNA
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177704-plasmid-map-sequence-id-352590

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  8. pDIV494

    Plasmid
    #177701
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177701-plasmid-map-sequence-id-352589

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  10. pDIV495

    Plasmid
    #177702
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177702-plasmid-map-sequence-id-353319

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  11. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Promoter
    TgTUB1 and Toxoplasma U6
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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  13. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75112-plasmid-map-sequence-id-305764

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  14. pDG330

    Plasmid
    #100898
    Purpose
    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100898-plasmid-map-sequence-id-195326

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  17. pX330 Mouse 3' Hp1a gRNA

    Plasmid
    #127898
    Purpose
    WT Cas9 Vector targeting the 3' end of the mouse Hp1a gene
    Depositor
    Mark Groudine
    Insert
    gRNA/Cas9
    Use
    CRISPR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127898-plasmid-map-sequence-id-251076

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  18. pX330 Mouse 5' Npm1 gRNA

    Plasmid
    #127900
    Purpose
    WT Cas9 Vector targeting the 5' end of the mouse Npm1 gene
    Depositor
    Mark Groudine
    Insert
    gRNA/Cas9
    Use
    CRISPR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127900-plasmid-map-sequence-id-249686

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  19. pDIV069

    Plasmid
    #247966
    Purpose
    CRISPR-Cas9 vector containing a 20 nt protospacer sequence to target the IS1 locus where the DIVERSIFY gene landing platform is integrated in Aspergillus nidulans. pyrG marker
    Depositor
    Uffe Mortensen
    Insert
    Cas9-AMA1-IS1-gRNA-pyrG-ori-AmpR
    Use
    CRISPR
    Mutation
    none
    Available Since
    March 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    247966-plasmid-map-sequence-id-503027

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  20. pUC57-ITR2-H1-GFP sgRNA Merry Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210743
    Purpose
    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 3
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Merry sgRNA GFP Target 3
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available Since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210743-plasmid-map-sequence-id-420083

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Showing: 4661 - 4680 of 14052 results