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RP hk339-GFP

Plasmid

#24431

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin WT (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

monomeric kinesin

Available Since

June 30, 2010

Availability

Academic Institutions and Nonprofits only

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pGLACTE-HT51-10

Plasmid

#99255

Purpose

Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. To generate capillary electrophoresis size standards.

Depositor

Karen Usdin

Article

Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi

Insert

FMR1 (FMR1 Human)

Tags

eGFP

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCK364.306

Plasmid

#192642

Purpose

Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

J306 scRNA

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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12URA-B-ScTop2∆CTD

Plasmid

#214924

Purpose

Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

Depositor

James Berger

Article

Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

Insert

topoisomerase II

Tags

6x His-TEV

Expression

Yeast

Mutation

Deleted amino acids 1178-1428

Promoter

Gal 1/10

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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Aurein1.2_(+36GFP)-Cre

Plasmid

#71748

Purpose

Aurein1.2+36GFP-Cre fusion expression in E. Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

(GGS)9_Aurein1.2+36GFP-Cre

Tags

6XHis

Expression

Bacterial

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pTRE2-Bla(HA–RILP)

Plasmid

#102425

Purpose

HA tag fused to the N-terminus of RILP for the expression in mammalian cells.

Depositor

Steven Weinman

Article

Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

Rab interacting lysosomal protein (RILP Human)

Tags

HA

Expression

Mammalian

Promoter

Tet-responsive

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAT9748-dCBE

Plasmid

#163008

Purpose

plasmid expressing CBE with dCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

dCBE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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AEB-No-aptamer-controlmRFP1

Plasmid

#63848

Purpose

This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

No-aptamer control mRNA

Use

Synthetic Biology

Promoter

J23100

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-Cx35-Halo-C-end

Plasmid

#71512

Purpose

Mammalian expression plasmid encoding perch Cx35 with a C-terminal HaloTag.

Depositor

John O'Brien

Article

Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

Insert

Connexin 35

Tags

HaloTag

Expression

Mammalian

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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p1071-Lenti-Single loxP

Plasmid

#217892

Purpose

Lentiviral vector, entry vector for Lentiviral Switch-OVER

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-intron

Plasmid

#177804

Purpose

An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

intron

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFosill-3

Plasmid

#89697

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarR2

Plasmid

#112813

Purpose

gateway cloning destination vector for expressing enhancer driven CD4-tdTomato

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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MSCVhygro-F-G9adSET

Plasmid

#41722

Purpose

Retroviral expression of human G9a mutant in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9adset (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mSynCAM 4

Plasmid

#115499

Purpose

expression of mouse full-length SynCAM 4

Depositor

Thomas Biederer

Article

Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

Insert

Cadm4 (Cadm4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-MCP-VPR-2AmScarlet

Plasmid

#121964

Purpose

Mammalian expression, Transcription regulation module

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

VP64-p65-Rta fusion module fused with MCP, recruited by MS2 hairpin

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRN3P_T3_ABEmax_IVT

Plasmid

#171761

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymerase

Depositor

Timo Otonkoski

Article

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Insert

ABEmax

Use

Vector for in-vitro transcription

Promoter

T3 promoter

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-E1.1-RFP

Plasmid

#22928

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

E1.1 binding site from Scardigli et al., 2003 with minimal CMV

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3_2FLAG-AKAP79ΔCN

Plasmid

#183451

Purpose

Expression of human AKAP79 lacking the calcineurin binding site (Δ337-343)

Depositor

Matthew Gold

Article

Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

Insert

AKAP5 (AKAP5 Human)

Tags

2xFLAG

Expression

Mammalian

Mutation

aa337-343 deleted

Promoter

CMV

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGLACTE-HT51-13

Plasmid

#99256

Purpose

Contains normal CGG repeats (29 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. To generate capillary electrophoresis size standards.

Depositor

Karen Usdin

Article

Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi

Insert

FMR1 (FMR1 Human)

Tags

eGFP

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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