We narrowed to 17,637 results for: URE
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Plasmid#174320PurposeExpression of recombinant GST-SLFN2-R67A, K68A, N71ADepositorInsertGST-SLFN2-R67A, K68A, N71A (Slfn2 Mouse)
UseBacmam, baculovirusExpressionMammalianPromoterCMVAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEZT-GST-SLFN2-T2
Plasmid#174321PurposeExpression of recombinant GST-SLFN2-K242A, K249A, Q301ADepositorInsertGST-SLFN2-K242A, K249A, Q301A (Slfn2 Mouse)
UseBacmam, baculovirusTagsFlagExpressionMammalianPromoterCMVAvailable SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcryGFP-acta1-dmdGFP
Plasmid#172530PurposealphaA-crystallin promoter (cry) drives GFP expression in the lens. The acta1 muscle-specific promoter drives expression of GFP-tagged zebrafish dmd.DepositorAvailable SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
R9-FS1
Plasmid#125208PurposeExpressing human Rad9b frameshift mutation found in a neural tube defect patientDepositorAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
R9-FS2
Plasmid#125209PurposeExpressing human Rad9b frameshift mutation found in a neural tube defect patientDepositorAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM10 with rat Δ192Annexin A11
Plasmid#164498PurposeFor bacterial expression of rat His-tagged Δ192Annexin A11DepositorInsertrat Δ192Annexin A11 (Anxa11 Rat)
TagsHisExpressionBacterialMutationN-terminal truncationPromoterT7Available SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM10 with rat Δ188Annexin A11
Plasmid#164497PurposeFor bacterial expression of rat His-tagged Δ188Annexin A11DepositorInsertrat Δ188Annexin A11 (Anxa11 Rat)
TagsHisExpressionBacterialMutationN-terminal truncationPromoterT7Available SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 delta787-843
Plasmid#166821PurposeGateway pDONR vector encoding NLRP1 with the patient mutation _787-843 (Zhong et al., 2016) (no stop codon)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Ub-NLRP1 S1213 P1214R
Plasmid#166830PurposepDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 T755N
Plasmid#166820PurposeGateway pDONR vector encoding NLRP1 with the patient mutation T755N (Drutman et al., 2019) (no stop codon)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 R726W
Plasmid#166819PurposeGateway pDONR vector encoding NLRP1 with the patient mutation R726W (Grandemange et al., 2017) (no stop codon)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim Actin MCH Puro (CAMP)
Plasmid#104446PurposeExpresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope. Puromycin resistant.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…PromoteractinAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHM080Env
Plasmid#139016PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein (env HIV-1)
ExpressionMammalianAvailable SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM075Env
Plasmid#139020PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein (env HIV-1)
ExpressionMammalianAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM141Env
Plasmid#139018PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein (env HIV-1)
ExpressionMammalianAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)
Plasmid#133791PurposeU6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018DepositorInsertSt1Cas9 sgRNA entry vector
ExpressionMammalianMutationV1 sgRNA architecture from Carter et al. biorxiv …PromoterU6Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)
Plasmid#133792PurposeU6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015DepositorInsertSt3Cas9 sgRNA entry vector
ExpressionMammalianMutationsgRNA architecture from Muller et al. Molecular T…PromoterU6Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM153BEnv
Plasmid#139023PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein (env HIV-1)
ExpressionMammalianAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-jph1a
Plasmid#109497PurposeMuscle specific zebrafish expression of mKate2 fused to junctophilin 1aDepositorInsertjph1a (jph1a Zebrafish)
UseZebrafish plasmidsTagsmKate2MutationPlease see Depositor CommentsAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM026Env
Plasmid#137681PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
ExpressionMammalianAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-ARF1-DN
Plasmid#109590PurposeMultisite gateway vector for 3' tagging with dominant negative ARF1DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-ARF6-DN
Plasmid#109592PurposeMultisite gateway vector for 3' tagging with dominant negative ARF6DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-SERCA1
Plasmid#109552PurposeMultisite gateway vector for 3' tagging with sarcoplasmic/endoplasmic calcium ATPase 1DepositorInsertsarcoplasmic/endoplasmic calcium ATPase 1 (atp2a1 Zebrafish)
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ARF1
Plasmid#109535PurposeMuscle specific zebrafish expression of mKate2 fused to ARF1DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-Cav3-mKate2
Plasmid#109518PurposeMuscle specific zebrafish expression of mKate2 fused to Caveolin3DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Dnm2a-DN
Plasmid#109520PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative Dynamin2aDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-EIF3B-88nt-Delta-Hairpin
Plasmid#136030PurposeEIF3B (465-552) 3'UTR with the main hairpin deleted inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-EIF3B-88nt-1st-Hairpin
Plasmid#136031PurposeEIF3B (465-552) 3'UTR with only the main hairpin inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-EIF3B-88nt-Disrupted-Hairpin
Plasmid#136032PurposeEIF3B (465-552) 3'UTR with the main hairpin mutated inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-EIF3B-88nt-Restored-Hairpin
Plasmid#136033PurposeEIF3B (465-552) 3'UTR with the main hairpin restored inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-EIF3B-88nt-A:U-Hairpin
Plasmid#136034PurposeEIF3B (465-552) 3'UTR with the main hairpin mutated to A and U nucleotides only inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTone-gata2aECE-nsGFP
Plasmid#132975Purposegata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backboneDepositorInsertsnuclear localized sfGFP
gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
UseUnspecified; Transposon-mediatedTags6x myc and SV40 NLSAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-TIRAP-cGASΔN-HA
Plasmid#130921PurposeExpresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection markerDepositorInsertTIRAP-ΔNcGAS
TagsHAExpressionMammalianMutationcGAS N terminus (aa1-159) replaced with the N ter…PromoterCMVAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_Ab-PirA
Plasmid#128959Purposeexpresses Acinetobacter baumannii PirA in E. coliDepositorInsertferric enterobactin receptor PirA
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-2xTS
Plasmid#117750PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialPromoterT7Available SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 RR837-8GG
Plasmid#113958Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-839, R837G and R838G substitutionPromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 R290A D291A T292A
Plasmid#112103PurposeMammalian expression of GFP-tagged Dynamin protein.DepositorAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406A M407A
Plasmid#112107PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406A M407A F409A
Plasmid#112108PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406R M407R
Plasmid#112109PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406R M407R F409A
Plasmid#112110PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47A)
Plasmid#112722PurposeArg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R47A)
Tags6His-TEVExpressionBacterialMutationR47APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47K)
Plasmid#112723PurposeArg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.DepositorInsert6His-TEV-TBP6.7(R47K)
Tags6His-TEVExpressionBacterialMutationR47KPromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48A)
Plasmid#112724PurposeGln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.DepositorInsert6His-TEV-TBP6.7(Q48A)
Tags6His-TEVExpressionBacterialMutationQ48APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(T50A)
Plasmid#112727PurposeLav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.DepositorInsert6His-TEV-TBP6.7(T50A)
Tags6His-TEVExpressionBacterialMutationT50APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R52A)
Plasmid#112729Purpose52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.DepositorInsert6His-TEV-TBP6.7(R52A)
Tags6His-TEVExpressionBacterialMutationR52APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(delta C-term)
Plasmid#112731PurposeThis mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.DepositorInsert6His-TEV-TBP6.7(delta C-term)
Tags6His-TEVExpressionBacterialMutationdelta C-termPromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_FNBP1-1/1
Plasmid#91552PurposeProtein expression and purification of human SH3 domain construct FNBP1-1/1DepositorInsertFNBP1-1/1 (FNBP1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ABL2-1/1
Plasmid#91533PurposeProtein expression and purification of human SH3 domain construct ABL2-1/1DepositorInsertABL2-1/1 (ABL2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CACNB2-2/2
Plasmid#91507PurposeProtein expression and purification of human SH3 domain construct CACNB2-2/2DepositorInsertCACNB2-2/2 (CACNB2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only