We narrowed to 30,760 results for: ple
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Plasmid#213265PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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Opto-GPR55
Plasmid#106026PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR55; Opto-GPR55; C4)DepositorInsertOpto-GPR55 (GPR55 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)
Plasmid#63139PurposeExpression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)DepositorTagsGSTExpressionBacterialMutationResidues 1-109 and Residues 1-159PromotertacAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-A3A-N57G
Plasmid#193639PurposeExpression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-hA3A-N57G, EGFP
ExpressionMammalianMutationhAPOBEC3A-N57GPromoterCMVAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-A3A-Y130F
Plasmid#193640PurposeExpression of base editor dCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-hA3A-Y130F, EGFP
ExpressionMammalianMutationhAPOBEC3A-Y130FPromoterCMVAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro
Plasmid#192682PurposeLentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcriptionDepositorInsertHuman ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFL His-SDS22 / PP1gamma
Plasmid#113511PurposeExpression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)DepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYpet-Optop38i
Plasmid#89749PurposeExpression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13FDepositorInsertOptop38i3
UseFluorescent proteinTagsYpetExpressionMammalianPromoterCMVAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
GPR141-DuET
Plasmid#213263PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Gal4-entry+NLS
Plasmid#128013PurposeVector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertGal4 DBD-SV40NLS-3x-Flag
UseGal4 entry vector with nls to generate tethering …TagsGal4-DBD-SV40NLS-3xFlagAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
GPR20-DuET
Plasmid#213275PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR1-DuET
Plasmid#213257PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-scrambled-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP
Plasmid#194897PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and scrambled crRNA without target in human genome.DepositorInsertsdLbCpf1-NLS-hCTCF_WT
U6-crRNA-scramble
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRA3 CTF-FLAG
Plasmid#217680PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRA3 (ADGRA3 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-737Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRA2 CTF-FLAG
Plasmid#217679PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRA2 (ADGRA2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-746Available SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR151
Plasmid#106051PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR151; Opto-GPR151; E5)DepositorInsertOpto-GPR151 (GPR151 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR18
Plasmid#106006PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR18; Opto-GPR18; A8)DepositorInsertOpto-GPR18 (GPR18 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG5 CTF-FLAG
Plasmid#217703PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG5 (ADGRG5 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-226Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB2 CTF-FLAG
Plasmid#217682PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB2 (BAI2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-911Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR27
Plasmid#106013PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR27; Opto-GPR27; B3)DepositorInsertOpto-GPR27 (GPR27 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only