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Plasmid#216742PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL2 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 2 (OL2) with the sequence of [LEDSGYMA…PromoterAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL4del
Plasmid#216744PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 4 (OL4) by examining the variant lacking OL4 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL5del
Plasmid#216745PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 5 (OL5) by examining the variant lacking OL5 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 5 (OL5) with the sequence of [ATFAA] i…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL1del
Plasmid#216741PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 1 (OL1) with the sequence of [INIGGALQ…PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Ex1-17-deltaP1P2-PTPN22
Plasmid#195391PurposeMammalian expression of a human PTPN22 mutant (exon 1-17 without the P1 and P2 domains) with FLAG tagDepositorInsertPTPN22 (PTPN22 Human)
UseTagsFLAGExpressionMammalianMutationPTPN22 mutant containing exon 1 to 17 and lacking…PromoterAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Ex1-17-deltaP2-PTPN22
Plasmid#195395PurposeMammalian expression of a human PTPN22 mutant (exon 1-17 without the P2 domain) with FLAG tagDepositorInsertPTPN22 (PTPN22 Human)
UseTagsFLAGExpressionMammalianMutationPTPN22 mutant containing exon 1 to 17 and lacking…PromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Ex1-17-deltaP1-PTPN22
Plasmid#195388PurposeMammalian expression of a human PTPN22 mutant (exon 1-17 without the P1 domain) with FLAG tagDepositorInsertPTPN22 (PTPN22 Human)
UseTagsFLAGExpressionMammalianMutationPTPN22 mutant containing exon 1 to 17 and lacking…PromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-chCAR-MycHis
Plasmid#87232PurposeMammalian expression of chicken CARDepositorInsertCXADR (CXADR Chicken)
UseTagsHis and MycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ2042.2_Brocc
Plasmid#140680PurposeBroccoli StripeDepositorInsertControl (“C”) lacking sg-1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL1del
Plasmid#216749PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
UseTags10x HisExpressionBacterialMutationOuter loop 1 (OL1) with the sequence of [FKISLAEM…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL2del
Plasmid#216750PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
UseTags10x HisExpressionBacterialMutationOuter loop 2 (OL2) with the sequence of [WMGILKNN…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL3del
Plasmid#216751PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 3 (OL3) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
UseTags10x HisExpressionBacterialMutationOuter loop 3 (OL3) with the sequence of [ELLGGPDS…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOpen-Endonuclease IV E. coli
Plasmid#165551PurposeDNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups.DepositorInsertEndonuclease IV E. coli
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
FTCAR2:pFlagCMV-mCAR-TVV
Plasmid#87239PurposeMammalian expression of mouse CAR, TVV isoformDepositorInsertCXADR (Cxadr Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10
Plasmid#219701PurposeLevel 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1DepositorInsertpAHP6::PhiC31-NLS:tUBQ1 + pGATA23::Bxb1-SAUR:tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
CNN3A-c007
Plasmid#175457PurposeProtein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.DepositorInsertCNN3 (CNN3 Human)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
kaiBC-
Plasmid#101826Purposedeletes kaiBC locusDepositorInsertCmR knocking out kaiBC operon
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
FTCAR1:pFlagCMV-chCAR-SIV
Plasmid#87238PurposeMammalian expression of chicken CAR, SIV isoformDepositorInsertCXADR (CXADR Chicken)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHD-DsRed-attP-w+
Plasmid#80898PurposeVector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.DepositorInsertsattP
LoxP-3xP3-DsRed-LoxP
GMR-white
UseHigh copy, amp resistanceTagsExpressionMutationPromoter3xP3 and GMR::hsp70Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET50_MDA5∆2CARD
Plasmid#216804PurposeExpresses melanoma differentiation-associated protein 5 lacking its 2 CARD domainsDepositorInsertmelanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
UseTagsExpressionBacterialMutation∆1-287PromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only