We narrowed to 11,748 results for: pcDNA

1. pcDNA3.1(+)-RasAR(R89L)-NES

   Plasmid

   #205568

   Purpose

   RasAR negative-control mutant plasmid. Targeted to cytosol.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   RasAR(R89L)-NES (RAF1 Synthetic, Human)
   

   Use

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Arg 89 mutated to Leu in Raf1 RBD region.

   Promoter

   CMV

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. OeNL(Ca2+)_18μ-pcDNA3

   Plasmid

   #111928

   Purpose

   Orange color bright luminescent calcium indicator with intermediate affinity

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   OeNL(Ca2+)_18μ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E67D, E104D, D133E at CaM

   Promoter

   CMV

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-Dest47- WTN23C11orf83-GFP

   Plasmid

   #65845

   Purpose

   Mammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

   Depositor

   Amos Bairoch

   Article

   Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

   Insert

   Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   WT Transmembrane

   Promoter

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT/TO-Hygro-3xFLAG

   Plasmid

   #185359

   Purpose

   Vector backbone for mammalian expression of proteins with an N-terminal 3xFLAG tag.

   Depositor

   Ayaz Najafov

   Insert

   3xFLAG
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG

   Plasmid

   #127322

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTR

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP Rab11a WT pcDNA3.1 Zeo (+)

   Plasmid

   #206079

   Purpose

   Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes

   Depositor

   Annette Dolphin

   Article

   Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

   Insert

   RAB11A (RAB11A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-f:PGC1(delta CTD)

   Plasmid

   #1030

   Depositor

   Bruce Spiegelman

   Article

   Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

   Insert

   PGC1a (Ppargc1a Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   delta CTD

   Promoter

   Available since

   Oct. 4, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/Hygro-Mfn2-ActA

   Plasmid

   #191981

   Purpose

   Expression of Mfn2 tagged with mitochondrion localization signal (ActA)

   Depositor

   Luca Scorrano

   Insert

   Mfn2-ActA
   

   Use

   Tags

   ActA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5/FRT/TO HIS DNAJB8

   Plasmid

   #19558

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Beta(4)Integrin-DeltaCYT

    Plasmid

    #16264

    Depositor

    Arthur Mercurio

    Article

    Clarke et al J Biol Chem. 1995 Sep 29. 270(39):22673-

    Insert

    Beta 4 Integrin (ITGB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    truncated cytoplasmic tail introduced a stop cod…

    Promoter

    Available since

    March 6, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pCDNA3_shRNA resistant PKA-Cα-mEGFP

    Plasmid

    #114143

    Purpose

    Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-FLAG-Lin28A-mCCHC

    Plasmid

    #51372

    Depositor

    Narry Kim

    Article

    Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

    Insert

    Lin28A (LIN28A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CCHC domain mutant (H147A, H169A)

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.2/V5 mmu-mir-375

    Plasmid

    #26336

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-375 (Mir375 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

14. RGS-6xHis-mRFP-pcDNA3.1-

    Plasmid

    #52516

    Purpose

    expresses mRFP in mammalian cells with RGS-6xHis tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    mRFP
    

    Use

    Tags

    RGS-6xHis

    Expression

    Mammalian

    Mutation

    engineered variant of tetrameric red fluorescent …

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)

    Plasmid

    #50031

    Purpose

    Expression of S. pyogenes SrtA at the plasma membrane in mammalian cells

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    PM-SrtA(strep)
    

    Use

    Tags

    C-HA

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. CeNL(Ca2+)_19μ-pcDNA3

    Plasmid

    #111927

    Purpose

    Cyan color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_19μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, F92W, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-puro-eGFP-HEC1-9A

    Plasmid

    #114023

    Purpose

    Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - 9A (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 9A

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. EphA2 430-976 pcDNA3.1

    Plasmid

    #102738

    Purpose

    Expression of c terminal EphA2 in mammalian cells

    Depositor

    Jin Chen

    Article

    Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

    Insert

    EphA2 N terminal (430-976aa) (Epha2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.2/V5 mmu-mir-137

    Plasmid

    #26327

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-137 (Mir137 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA 3.1(-) mouse Egr4

    Plasmid

    #108096

    Purpose

    Expresses mouse Egr4 in mammalian cells

    Depositor

    Peter Johnson

    Article

    Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

    Insert

    Egr4 (Egr4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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