We narrowed to 10,490 results for: nar
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FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
Plasmid#137830PurposeMESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factorDepositorInsertFRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
UseTags3x FLAGExpressionMammalianMutationMutated Leucine 71 to Lysine in CTEVp fragmentPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK569
Plasmid#110614PurposeBTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoterDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationV32M (see comments below)PromoterAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(E17K)
Plasmid#86563PurposeExpresses human full-length Akt1(E17K) mutantDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationE17K mutation in the PH domainPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO(ChRger2-TS-YFP)
Plasmid#127239PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterCAGAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB122
Plasmid#68576PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
YFP Rab4DN
Plasmid#99019PurposeLocalization and visualization of the dominant negative form of Rab4DepositorInsertRab4 DN (Rab4 Synthetic, Fly)
UseTagsYFPExpressionMammalianMutationS22N Dominant negativePromoterCMVAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7A
Plasmid#62284Purposemammalian expression of human duplicated Alpha7 (CHRFAM7A)DepositorInsertCHRFAM7A (CHRFAM7A Human)
UseTagsExpressionMammalianMutationpartial duplication of CHRNA7PromoterCMVAvailable sinceMarch 3, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQZ210
Plasmid#106414PurposeCo-expresses trans SNARE complex with pDC183 in E.coliDepositorInsertsUseTagsHis tagExpressionBacterialMutationFragment 191-256 , Codon optimization and Fragmen…PromoterT7Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-eYFP-3x-miR708-5p-TS
Plasmid#117381PurposeAn AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neuronsDepositorInsertEYFP + 3 copies of miR708: CCCAGCTAGATTGTAAGCTCCTT
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNRDc-E>A-V5
Plasmid#51298Purposeexpression of the inactive mutant of mouse NRDc in mammalian cellsDepositorInsertNRDc E247A (Nrd1 Mouse)
UseTagsHIS and V5ExpressionMammalianMutationE247A, enzymatic inactive mutantPromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJM529 EF1α 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA in TUPV5
Plasmid#161555PurposeConstitutive expression of 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA under the EF1α promoterDepositorInsert3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA
UseSynthetic BiologyTags3x-FLAG and HAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK614
Plasmid#110608PurposeBTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)DepositorInsertdCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG-eYFP-3x-miR204-5p-TS
Plasmid#117380PurposeAn AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytesDepositorInsertEYFP + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK601
Plasmid#110607PurposeBTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)DepositorInsertCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB321
Plasmid#68649PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-PH-citrine
Plasmid#131406PurposePurpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrineDepositorInsertPH
UseIn vitro transcriptionTagscitrine yellow fluorescent proteinExpressionMutationPromoterT7Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB119
Plasmid#68573PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt1(D323A/D325A)
Plasmid#86630PurposeExpresses eGFP-tagged human Akt1(D323A/D325A) mutantDepositorInsertAkt1(D323A/D325A) (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDC183
Plasmid#106415PurposeCo-expresses trans SNARE complex with pQZ210 in E. coliDepositorInsertsUseTagsExpressionBacterialMutationFragment 141-204, Codon optimization and Fragment…PromoterT7Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only
