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PAS533 (TJF076)

Plasmid

#65607

Purpose

pETDuet-1 Containing FadD V451A-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Tags

6x His

Expression

Bacterial

Mutation

V451A

Promoter

T7 lacO

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
PAS512 (TJF032 S377R)

Plasmid

#65579

Purpose

pETDuet-1 Containing FadD S377R

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Expression

Bacterial

Mutation

S377R

Promoter

T7 lacO

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
PAS510 (TJF032 G446S)

Plasmid

#65577

Purpose

pETDuet-1 Containing FadD G446S

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Expression

Bacterial

Mutation

G446S

Promoter

T7 lacO

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pHL 342

Plasmid

#53012

Purpose

PLtetO-1:mCherry, PLlacO-1:araC, Plar:ompC::gfp

Depositor

Han Lim

Article

Hussein et al Nucleic Acids Res. 2012 May 22.

Inserts

mCherry
araC
ompC

Use

Synthetic Biology

Tags

gfp

Expression

Bacterial

Mutation

contains -81 to +36 relative to start codon

Promoter

Plar, pLlacO-1, and pLtetO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pHL1506

Plasmid

#53026

Purpose

PLlacO-1:RBS(st7):csrA

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Insert

csrA

Use

Synthetic Biology

Tags

RBS(st7)

Expression

Bacterial

Promoter

pLlac0-1

Available Since

May 13, 2014

Availability

Academic Institutions and Nonprofits only
pHL1318

Plasmid

#37637

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

TetR
CsrA

Use

Synthetic Biology

Expression

Bacterial

Promoter

pConNoHind

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only
pSL5 SMR1713

Plasmid

#28028

Depositor

Susan Rosenberg

Article

Harris et al Genes Dev. 1997 Sep 15. 11(18):2426-37.

Insert

MutL (mutL E. coli)

Expression

Bacterial

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
pJF6

Plasmid

#21678

Depositor

James Haber

Article

Fishman-Lobell et al Mol Cell Biol. 1992 Mar . 12(3):1292-303

Insert

LacZ (lacZ E. coli)

Use

Centromeric plasmid

Mutation

HO digestion site in one of the LacZ genes

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pDL11c

Plasmid

#17833

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

alcA promoter, lacZ, argB_1626,

Use

Synthetic Biology; Aspergillus nidulans expression

Mutation

The argB_1626 mutation consists of two sequential…

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pRSET FLII282Pglu-4m

Plasmid

#13564

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A T282S-ECFP-N283R

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pESdelta 799

Plasmid

#13042

Depositor

Peggy Hsieh

Article

Biswas et al J Mol Biol. 2001 Jan 26. 305(4):805-16.

Insert

MutS

Tags

His6

Expression

Bacterial

Mutation

C-terminal deletion from aa 800 through 853.

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
Nuclear PancACe

Plasmid

#215706

Purpose

Expresses acetyl-CoA biosensor in the nucleus

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and NLS

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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Cytoplasmic PancACe

Plasmid

#215708

Purpose

Expresses acetyl-CoA biosensor in the cytoplasm

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and cytoplasmic localization sequence

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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Mitochondrial PancACe

Plasmid

#215710

Purpose

Expresses acetyl-CoA biosensor in the mitochondria

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and mitochondrial localization sequence…

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-Ec1

Plasmid

#138474

Purpose

Enables high-efficiency recombineering in E. coli

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

CspRecT
mutL E32K

Expression

Bacterial

Mutation

E32K

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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secreted BirA-Flag

Plasmid

#64395

Purpose

Flag-tagged biotinylation enzyme for co-expression with bio-proteins

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

BirA (birA E. coli)

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pET30a-AlkB-D135S

Plasmid

#79051

Purpose

Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

Depositor

Tao Pan

Article

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET30a-AlkB

Plasmid

#79050

Purpose

Express wild type AlkB protein in E. coli BL21(DE3)

Depositor

Tao Pan

Article

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBMN DHFR(DD)-YFP

Plasmid

#29325

Depositor

Thomas Wandless

Article

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

DHFR(DD)-YFP

Use

Retroviral

Tags

HA and YFP

Mutation

Change Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

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pET21-Streptavidin-K121R

Plasmid

#89880

Purpose

Streptavidin that has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Article

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K121R-Glutamate_Tag

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K121R

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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