We narrowed to 9,823 results for: 158
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pLKO.1-Tet-Puro-shMMERVK10c_1 (inducible)
Plasmid#189955PurposeshRNA mediated knockdownDepositorInsertERV_MMERVK10c
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSR357.9
Plasmid#125105PurposeExpression of narL(1-129aa)-ydfI(124-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)129
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Hygro-sgTom
Plasmid#138698PurposeExpresses a tomato-targeting sgRNA and Cas9DepositorInsertsgTomato
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pk_CyRPA-Cd4-bio-His
Plasmid#126841PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertCyRPA (PKH_052740 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM3D(Gq)::RAM-d2tTA
Plasmid#189627PurposeExpresses hM3D(Gq) under the hSyn promoter, and d2tTA under the pRAM (synthetic Fos) promoter. Plasmid 1 for recording Fos expression.DepositorInsertshM3D(Gq)
d2tTA
UseAAVTagsHAExpressionMammalianMutationPromoterhSyn and pRAMAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
[pSK475] pLJC5 HA-Depdc5(A)
Plasmid#109344PurposeLenti-viral expression of HA-tagged Depdc5 mutant ADepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsHAExpressionMutationDIYGD(185-189)GSGSGPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
[pSK476] pLJC5 HA-Depdc5(B)
Plasmid#109345PurposeLenti-viral expression of HA-tagged Depdc5 mutant BDepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsHAExpressionMutationEQPLH(371-375)GSGSGPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
[pSK478] pLJC5 HA-Depdc5(P)
Plasmid#109347PurposeLenti-viral expression of HA-tagged Depdc5 mutant PDepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsHAExpressionMutationYDLLP(775-779)GSGSGPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBK.009
Plasmid#187209PurposeExpresses retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG]), and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG])
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Ptger1
Plasmid#170306PurposeA knock-out vector for the mouse Ptger1DepositorInsertA gRNA targeting the mouse Ptger1 gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
QQR1 variant
Plasmid#138564PurposeExpresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSDDepositorInsertQQR1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationG1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
DsRed +9e
Plasmid#191766PurposeExpression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9eDepositorInsertDsRed
UseTagsN terminal fusion of positively charged polypetod…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP-YPet-RanGAP*
Plasmid#175245PurposeBacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPetDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plxnc1-Fc-His
Plasmid#72131PurposeExpresses the extracellular region of the PlexinC1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxnc1 (Plxnc1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
CABE-T2.6
Plasmid#193263PurposeExpression of CABE-T2.6 base editor in mammalian cells, CMV promoterDepositorInsertCABE-T2.6
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CBE-T1.6
Plasmid#193275PurposeExpression of CBE-T1.6 base editor in mammalian cells, CMV promoterDepositorInsertCBE-T1.6
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL0721 (pDonor_L1-105)
Plasmid#130645PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.DepositorInsertVchCAST donor DNA (short L)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
ABE8e(TadA-8e K20A R21A)
Plasmid#138496PurposeA-to-G base editorDepositorInsertecTadA(8e K20A R21A)-nSpCas9
UseTagsExpressionMammalianMutationTadA-8e K20A R21APromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL0376 (His-MBP-StrepII-TniQ)
Plasmid#130641PurposeExpresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.DepositorInsertVchTniQ
UseCRISPR; TransposonTagsHis10-MBP-TEVsite-StrepII on TniQExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL0715 (pDonor_R1-57)
Plasmid#130644PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.DepositorInsertVchCAST donor DNA (short R)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
VRER variant
Plasmid#138561PurposeExpresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSDDepositorInsertVRER
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Ptgs2_Balbc
Plasmid#170301PurposeA knock-out vector for the mouse Ptgs2 in Balb/cDepositorInsertA gRNA targeting the mouse tyrosinase gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXW117As(RS-E11)
Plasmid#123145PurposeArsenic sensor with two-layered amplifier cascade. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-tDepositorInsertJ117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ117, ParsR, PhrpLE, Pecf11Available sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Gnaq
Plasmid#170302PurposeA knock-out vector for the mouse GnaqDepositorInsertA gRNA targeting the mouse Gnaq gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCarCBAE
Plasmid#117755PurposeCodon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backboneDepositorInsertCodon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
UseTagsExpressionBacterialMutationPromoterPLac(UV5)Available sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
WPXL LEFTY2 gRNA
Plasmid#101924PurposeLEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.DepositorInsertLEFTY2 enhancer targeting gRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKD265
Plasmid#125095PurposeExpression of torR(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertstorR(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR322
Plasmid#125116PurposeExpression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsuhpA(REC)-ydfI(DBD)131
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.15
Plasmid#125082PurposeExpression of ompR(1-137aa)-ccaR(127-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only
