We narrowed to 18,890 results for: Tec

1. pPANI2A2E2-Crimson

   Plasmid

   #193758

   Purpose

   Expresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

   Insert

   E2-Crimson
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PANI2 synthetic promoter carrying binding sites f…

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-5xUASG-iRFP-B3-timestamp-A

   Plasmid

   #159925

   Purpose

   timestamp Template A inserted into the 3’ UTR of iRFP, in a second generation lentiviral backbone with the Vivid promoter, with RNA barcode CTG. AKA 149B3.

   Depositor

   Fei Chen

   Article

   Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

   Insert

   iRFP-Timestamp A
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBT371

   Plasmid

   #125612

   Purpose

   Expresses CDA1-BE4max-NG in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   CDA1-BE4max-NG
   

   Expression

   Mammalian

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYPQ210

   Plasmid

   #109329

   Purpose

   Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Type

   Empty backbone

   Use

   Gateway compatible attr1-attr2 destination vector

   Expression

   Plant

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGY53

   Plasmid

   #221709

   Purpose

   Empty Cas12a vector for multiplex gene editing in fungi

   Depositor

   Kyle Pomraning

   Article

   Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

   Type

   Empty backbone

   Use

   CRISPR; Aspergillus

   Expression

   Bacterial

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTS1017-Tier1-OTetO7-PCMVmin-SEAP

   Plasmid

   #169586

   Purpose

   Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP expression
   

   Expression

   Mammalian

   Promoter

   TetO7-PCMVmin

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Gata3-T2A-H2B-eGFP donor

   Plasmid

   #113119

   Purpose

   Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Gata3 donor region including homology arms and T2A-H2B-eGFP
   

   Use

   Synthetic Biology

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Fgfr2-T2A-H2B-Halo donor

   Plasmid

   #113117

   Purpose

   Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Fgfr2 donor region including homology arms and T2A-H2B-Halo
   

   Use

   Synthetic Biology

   Available Since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pISFba1

   Plasmid

   #226820

   Purpose

   Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 ori

   Depositor

   Sheng Yang

   Article

   Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

   Insert

   ISFba1
   

   Expression

   Bacterial

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pISFba1reRNA

    Plasmid

    #226822

    Purpose

    Expressing ISFba1 guide RNA targeting maeB site controled by PJ23119

    Depositor

    Sheng Yang

    Article

    Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

    Insert

    ISFba1 none target guide RNA
    

    Expression

    Bacterial

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKB817 (pBra-Hfq)

    Plasmid

    #174661

    Purpose

    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.

    Depositor

    Katherine Berry, Ann Hochschild

    Article

    Berry et al Nucleic Acids Res. 2018 Jan 25;46(2):e12

    Insert

    Hfq
    

    Tags

    alpha NTD

    Expression

    Bacterial

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PclpB riboswitch GFP pZa

    Plasmid

    #170078

    Purpose

    GFP expression under the control of E. coli clpB promoter engineered with riboregulators

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PclpB

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-Cas9-NRRH

    Plasmid

    #136924

    Purpose

    Expresses Cas9-NRRH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRRH
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-iCre

    Plasmid

    #125577

    Purpose

    Expresses iCre under the CAG promoter in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    iCre
    

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCellFree_G03 WAVE 2

    Plasmid

    #67098

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    WAVE 2 (WASF2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pB-dCas9-PBE

    Plasmid

    #98159

    Purpose

    Expresses CRISPR base editor in maize

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-dCas9-UGI-NLS
    

    Use

    Maize

    Expression

    Plant

    Promoter

    Ubi

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKB083

    Plasmid

    #181952

    Purpose

    IPTG-inducible expression of PhoQ

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    PhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)
    

    Expression

    Bacterial

    Promoter

    PhoQ-Ptac; lacI-PlacIq

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Fragment 1 Null

    Plasmid

    #198729

    Purpose

    Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    gRNA expression cassette in pENTR4
    

    Use

    Gateway-compatible entry vector

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmKalama1-Nuc

    Plasmid

    #14894

    Purpose

    Mammalian expression of mKalama1 blue fluorescent protein with NLS

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 May 22;46(20):5904-10

    Insert

    pmKalama1-Nuc
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    I178T mismatch, replace EYFP of the vector pEYFP-…

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. p35S-A3A-PBE

    Plasmid

    #119773

    Purpose

    Conversion of C to T in potato

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    CaMv 2x35S

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge