We narrowed to 16,541 results for: puromycin

1. Fireworks GFP[PTC+] (AVA2600)

   Plasmid

   #85446

   Purpose

   Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

   Depositor

   Joan Steitz

   Article

   Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

   Insert

   tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
   

   Use

   Minimal backbone fragment for low-copy bacterial …

   Expression

   Mammalian

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. DKC1 F1.3 gRNA

   Plasmid

   #90652

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DKC1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   DKC1 (Guide Designation F1.3)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE-puro LIP

   Plasmid

   #15713

   Depositor

   Joan Massague

   Article

   Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

   Insert

   LIP (CEBPB Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

4. LentiCRISPRv2-KLF7-1

   Plasmid

   #169797

   Purpose

   Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

   Depositor

   Natsuko Chiba

   Article

   Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

   Insert

   guide RNA for KLF7 neighbouring region
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. DKC1 F2.3 gRNA

   Plasmid

   #90653

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DKC1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   DKC1 (Guide Designation F2.3)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONOR-tagBFP-PSM-EGFP

   Plasmid

   #100603

   Purpose

   Donor scaffold with EGFP positive selection module, and tagBFP negative selection module

   Depositor

   Jens Schwamborn

   Article

   Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   EGFP-T2A-Puro and tagBFP

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-puro-TTF-1-wt

   Plasmid

   #74990

   Purpose

   Retroviral vector holding the human wild-type full-length TTF-1 (NKX2-1) cDNA

   Depositor

   David Mu

   Article

   Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

   Insert

   TTF-1 (TTF1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   wild-type, full-length, without 3' UTR

   Available Since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2T-CAG-MCS-P2A-GFP-PuroR

   Plasmid

   #107186

   Purpose

   Base plasmid for cloning gene duplication alleles with eGFP reporter.

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Type

   Empty backbone

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5FRT PUR mGSK3A K148A

   Plasmid

   #35158

   Depositor

   Jim Woodgett

   Article

   Doble et al Dev Cell. 2007 Jun;12(6):957-71.

   Insert

   GSK-3α (Gsk3a Mouse)
   

   Expression

   Mammalian

   Mutation

   K148A (dominant negative)

   Promoter

   CMV

   Available Since

   May 18, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

10. FEN1 C10.3 gRNA

    Plasmid

    #90689

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FEN1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    FEN1 (Guide Designation C10.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiGuide-RRBP1-2

    Plasmid

    #92157

    Purpose

    CRISPR guide RNA targeting human RRBP1

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    RRBP1 sgRNA-2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

    Plasmid

    #197067

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    MitoTrap-mCherry
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRetroSuper-shFyn

    Plasmid

    #26985

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    shRNA Fyn (FYN Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pLib6.4-Aalb_743

    Plasmid

    #176662

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXspuro-GFP-NBR1-dLIR

    Plasmid

    #74204

    Purpose

    Retroviral expression of GFP-NBR1 with aa 727-738 deletion

    Depositor

    Jayanta Debnath

    Article

    Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

    Insert

    neighbor of BRCA1 gene 1 (NBR1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 727-738

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pSLQ1371-sgGAL4

    Plasmid

    #121514

    Purpose

    sgGAL4 control. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    sgGAL4
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-HIF2α P531A-pBabe-puro

    Plasmid

    #26091

    Depositor

    William Kaelin

    Article

    Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA with point mutation P531A

    Available Since

    Sept. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH_SBP-mCherry-Ecadherin

    Plasmid

    #65291

    Purpose

    Ecadherin reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Lentiviral

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiCRISPR-sgPREX1

    Plasmid

    #86127

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting PREX1

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    gRNA targeting PREX1 (PREX1 )
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. BII-ChPtW-EGFP

    Plasmid

    #133400

    Purpose

    Piggybac vector for constitutive expression of EGFP

    Depositor

    Blair Madison

    Article

    Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

    Insert

    EGFP
    

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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