162,590 results

1. qTAG-C-miRFP670nano3-Blast-H3C2

   Plasmid

   #207784

   Purpose

   Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pRK5-FLAG-SZT2

   Plasmid

   #87034

   Purpose

   mammalian expression of SZT2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   SZT2 (SZT2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX333

   Plasmid

   #64073

   Purpose

   Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

   Depositor

   Andrea Ventura

   Article

   Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   3XFLAG-Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CBh; U6

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVITRO1-Trastuzumab-IgG3/κ

   Plasmid

   #61885

   Purpose

   Expresses HER2/neu receptor specific humanized IgG3/κ antibody isotype (Trastuzumab-IgG3/κ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   HER2/neu receptor specific humanized Gamma 3 heavy chain expression cassette
   HER2/neu receptor specific humanized kappa light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mEF1 Prom and rEF1 Prom

   Available since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CMV-mOC-STAMP-GFP

   Plasmid

   #73579

   Purpose

   Lentivirus expression of mOC-STAMP

   Depositor

   Paul Odgren

   Article

   Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

   Insert

   OC-STAMP (Ocstamp Mouse)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-ExRai-AMPKAR

   Plasmid

   #192446

   Purpose

   Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   ExRai-AMPKAR
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-PR rep/cap

   Plasmid

   #197565

   Purpose

   encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

   Depositor

   Casey Maguire

   Article

   Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

   Inserts

   AAV Rep genes
   AAV9 VP1 Cap gene with PR insert
   

   Use

   AAV

   Tags

   Expression

   Mutation

   contains 7mer insert PRPPSTH between amino acids …

   Promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_PPARG_WT

   Plasmid

   #82223

   Purpose

   Gateway Donor vector containing PPARG , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PPARG (PPARG Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. siRNA-resistant ss-SigmaR1DTM-mNeonGreen R119A F191A

   Plasmid

   #226576

   Purpose

   Encodes mutant siRNA resistant SigmaR1 protein (transmembrane region deletion + substitutions) labeled with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   R119A, F191A

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. ss-SigmaR1DTM-mNeonGreen

    Plasmid

    #226575

    Purpose

    Encodes mutant SigmaR1 protein (transmembrane region deletion) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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