We narrowed to 14,982 results for: egf
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Plasmid#172100PurposeMammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-eDHFR(69K6)-20aa
ExpressionMammalianMutationeDHFR(69K6): Hexalysine (K6) sequence is inserted…PromoterCMVAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC150-RFP-EGFP-LGALS3 R186S
Plasmid#175779PurposePiggyBac Vector expressing GFP-RFP-LGALS3 R186SDepositorAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA
Plasmid#178827PurposeCre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoterDepositorInsertSomQuasAr6a_EGFP-P2A-somCheRiff_HA
UseAAV, Cre/Lox, and Mouse TargetingTagsEGFP, HAExpressionMammalianPromoterhSynAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP
Plasmid#172670PurposeFor expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition siteDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSDHB pegRNA and SDHB_nick-sgRNA
ExpressionMammalianPromoterU6Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1(A322D)-IRES2-EGFP
Plasmid#170821Purposehuman GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuman GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
TagsIRES2-EGFPExpressionMammalianMutationchange Alanine 322 to AspartateAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP
Plasmid#154303PurposeExpresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideumDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertshistone H1
γ-tubulin
UseDictyostelium expressionTagsmEGFP and mRuby3Promoteractin15Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB-6HIS-TEV-mEGFP,IRES-NGFR
Plasmid#164521PurposeAll-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneExpressionMammalianAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
R26-EGFP MMEJ donor vector
Plasmid#137926PurposeR0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSA-EGFP-bpA with micro-homology arms
UseMouse TargetingAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa (T305D/T306D)
Plasmid#127611PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T305D/T306D)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
TagsmEGFPExpressionMammalianMutationchanged Threonine 305/306 to AlaninePromoterpCAGAvailable sinceJan. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-gal4-VP16-gcry-eGFP
Plasmid#117779PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgal4/VP16
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
BE3(E63Q)-P2A-EGFP (pRZ189)
Plasmid#123613PurposeCAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).DepositorInsertBE3(E63Q)-P2A-EGFP
ExpressionMammalianMutationE63Q in rAPOBEC1, D10A in SpCas9PromoterCAGAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP Δnef/U3
Plasmid#101346PurposeDeletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequencesDepositorInsertNL4-3
UseLentiviralMutationdelta nef/LTRAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2
Plasmid#75295PurposeAn AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.DepositorAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucEGFPmiR-mCherry-TEV
Plasmid#105808PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal mCherry.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsmCherry-TEVExpressionMammalianAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucEGFPmiR-TEV-mCherry
Plasmid#105812PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-mCherryExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBac{3XP3::EGFP, Pactin::Ptrsps}
Plasmid#86861PurposeExpresses EGFP in eyes and ubiquitous expression of P-transposaseDepositorInsertpACNNTNPII-S129A
ExpressionInsectAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCALNL-eGFP-NT1-6-gix-6-NT1
Plasmid#81204Purposecontains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmidDepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
ExpressionMammalianPromoterpCBaAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP TEM4 DEL 125-135
Plasmid#58896PurposeExpresses GFP tagged full length TEM4 protein with amino acids 125 - 135 deletedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTEM4 (ARHGEF17 Human)
TagsEGFPExpressionMammalianMutationDeleted aa's 125-135PromoterCMVAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/CGA-mEGFP-IRES-mCherry
Plasmid#49233PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/UAA-mEGFP-IRES-mCherry
Plasmid#49225PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only