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pWT016b

Plasmid

#96853

Purpose

dHHR-bsgRNA-GFP (13 nt blocker)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

dHHR-bsgRNA-GFP (13 nt blocker)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJOG292

Plasmid

#80586

Purpose

empty vector (lacZ) for 2nd generation recipient assembly

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Type

Empty backbone

Expression

Plant

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG294

Plasmid

#80587

Purpose

dummy module (ccdB) for 2nd generation recipient assembly

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR207-Citrine-PTS1

Plasmid

#44588

Purpose

Gateway entry vector for Citrine fluorescent protein with a peroxisome localization signal

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-PTS1

Tags

PTS1 (peroxisomal targeting signal type 1)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS292

Plasmid

#73725

Purpose

SapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

2xNLS-mCherry + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pEG795

Plasmid

#40122

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (ATG through 648 bp of 3'UTR) (RNAi resistant exon 2) (mex-5 Nematode)

Use

Gateway donor vector

Mutation

Exon 2 recoded to be RNAi-resistant

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pBS ZfHoxa9a

Plasmid

#27518

Depositor

Victoria Prince

Article

Prince et al Development. 1998 Feb . 125(3):407-20.

Insert

Hoxa9a (hoxa9a Zebrafish)

Use

Cloning vector

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
pDP-let-7_wt

Plasmid

#26558

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7

Use

Transcription vector

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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RCAS(A) cTbx5 D1 (CT#636)

Plasmid

#13968

Depositor

Cliff Tabin

Article

Rallis et al Development. 2003 Jun . 130(12):2741-51.

Insert

Tbx5 (TBX5 Chicken)

Use

Retroviral; Avian expression

Mutation

Amino acids 1-274

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pFUW H2B-mTagBFP2-P2A-PuroR

Plasmid

#239552

Purpose

Expression and nuclear localization of mTagBFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mTagBFP2

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-mCherry-NLS

Plasmid

#87695

Purpose

Gal4VP16 driven expression of mCherry-NLS in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

mCherry-NLS

Tags

nuclear localization signal

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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LGmCreER (self deleting)

Plasmid

#33340

Depositor

Scott Lowe

Article

Zhao et al Genes Dev. 2010 Jul 1;24(13):1389-402.

Insert

CreERT2

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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PPRE X3-TK-luc

Plasmid

#1015

Purpose

PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

Depositor

Bruce Spiegelman

Article

Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

Insert

PPRE

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

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pFUW mTagBFP2-P2A-PuroR

Plasmid

#239565

Purpose

Cytosolic expression of mTagBFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mTagBFP2

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-Tol2 transposase

Plasmid

#158774

Purpose

Expression of Tol2 transposase

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

Tol2 tranposase

Expression

Mammalian

Mutation

Tol2 codon usage frequency well suitable for muri…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-TRE3G-dCas9-HaloTag

Plasmid

#169448

Purpose

expression of dCas9-HaloTag

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

dCas9-HaloTag

Use

Lentiviral

Expression

Mammalian

Available Since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFUW mito-GRX1-roGFP2

Plasmid

#239561

Purpose

Expression and mitochondrial localization of Grx1-roGFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

roGFP2

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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4 gRNA concatemer

Plasmid

#84881

Purpose

Empty concatmer vector in which 4 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pR-spondin 1

Plasmid

#128844

Purpose

bacterial expression of pR-spondin 1

Depositor

Marc de la Roche

Article

Urbischek et al Sci Rep. 2019 Apr 17;9(1):6193. doi: 10.

Insert

R-spondin 1 (RSPO1 Human)

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pFUW H2B-mEmerald-P2A-PuroR

Plasmid

#239553

Purpose

Expression and nuclear localization of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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