We narrowed to 4,011 results for: plasmid lenti crispr

1. pLenti-NRCH-PE2max-BSD

   Plasmid

   #191103

   Purpose

   Lentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   NRCH-PEmax
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed R221K, N394K, H840A from SpCas9-NRCH

   Promoter

   EF-1a

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiRCas9-Lambda2

   Plasmid

   #104184

   Purpose

   Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

   Depositor

   Eugene Yeo

   Article

   Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

   Insert

   U6-sgRNA, EFS-PIN-dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiUniversal-Puro

   Plasmid

   #127749

   Purpose

   Lentiviral plasmid for expressing any U6 driven transcripts (sgRNAs, crRNAs, shRNAs and etc...)

   Depositor

   Giulio Draetta

   Article

   Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide Puro-P2A-EGFP

   Plasmid

   #137729

   Purpose

   For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

   Depositor

   Fredrik Wermeling

   Article

   Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti_PGK-Cas9-P2A-HygR

   Plasmid

   #164135

   Purpose

   Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   SpCas9 (cas9 Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti_EFS-Cas9-P2A-HygR

   Plasmid

   #164134

   Purpose

   Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   SpCas9 (cas9 Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti_CMV-Cas9-P2A-HygR

   Plasmid

   #164133

   Purpose

   Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   SpCas9 (cas9 Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOT_7-lenti-EFS-SS1-BBz-2A-puro

   Plasmid

   #217990

   Purpose

   Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Xue et al bioRxiv 2024.11.27.625752

   Insert

   SS1-BBz CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-dCas9-KRAB-blast

   Plasmid

   #89567

   Purpose

   Plasmid expression dCas9 protein in fusion with KRAB domain

   Depositor

   Gary Hon

   Article

   Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   Blasticidin resistance gene and KRAB

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL7 lenti-dcas9-3xflag-blast

    Plasmid

    #112133

    Purpose

    lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    dCas9(D10A, N863A)-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A mutants in Cas9

    Promoter

    EF1a

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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