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Addgene

We narrowed to 3,499 results for: Braf;

Showing: 461 - 480 of 3499 results
  1. pMT-ubb-Avi-Cerulean-Rpl10a

    Plasmid
    #79882
    Purpose
    Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences
    Insert
    Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
    Use
    Tags
    Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pMTB2-Avi-Cerulean-Rpl10a

    Plasmid
    #79880
    Purpose
    Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences
    Insert
    Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
    Use
    Tags
    Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. pT22Ubm4Eng2bKRPC(#493)

    Plasmid
    #184091
    Purpose
    cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic
    Depositor
    Insert
    4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    4xMyc
    Expression
    Mutation
    ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…
    Promoter
    Zf-Ubi
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pT3TS-zSpG

    Plasmid
    #179316
    Purpose
    In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter
    Insert
    Zebrafish codon-optimized Cas9 variant SpG
    Use
    CRISPR; In vitro transcription by t3 promoter
    Tags
    SV40-NLS
    Expression
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R
    Promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pT3TS-zSpRY

    Plasmid
    #179317
    Purpose
    In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter
    Insert
    Zebrafish codon-optimized Cas9 variant SpRY
    Use
    CRISPR; In vitro transcription by t3 promoter
    Tags
    SV40-NLS
    Expression
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…
    Promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry

    Plasmid
    #230043
    Purpose
    Partial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.
    Depositor
    Insert
    lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR
    Use
    Cre/Lox; Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    fabp10a
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid
    #135766
    Purpose
    This plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.
    Insert
    Wilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
    Use
    Zebrafish
    Tags
    Expression
    Mutation
    Promoter
    UAS
    Available since
    Jan. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pSYC-201

    Plasmid
    #178963
    Purpose
    Human GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombination
    Depositor
    Inserts
    Glial fibrillary acidic protein promoter (gfap Zebrafish)
    Glial fibrillary acidic protein (GFAP Human)
    EGFP-pA
    Use
    Tol2 mediated recombination
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    zebrafish gfap promoter
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pSYC-204

    Plasmid
    #178964
    Purpose
    Human GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombination
    Depositor
    Inserts
    Glial fibrillary acidic protein promoter (gfap Zebrafish)
    Glial fibrillary acidic protein (GFAP Human)
    EGFP-pA
    Use
    Tol2 mediated recombination
    Tags
    3xFLAG
    Expression
    Mutation
    R79C
    Promoter
    Zebrafish gfap promoter
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. ZE22 vox-CS (ZV100)

    Plasmid
    #17064
    Depositor
    Insert
    vox-2 (vox Zebrafish)
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2008
    Availability
    Academic Institutions and Nonprofits only
  11. ZE49 pSPORT1-Sp5-l

    Plasmid
    #17090
    Depositor
    Insert
    Sp5l (sp5l Zebrafish)
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2008
    Availability
    Academic Institutions and Nonprofits only
  12. pDONR221-mis18a

    Plasmid
    #192747
    Purpose
    Gateway entry vector encoding zebrafish mis18a
    Depositor
    Insert
    mis18a (si:dkey-189i22.1 Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pDONR221-chodl

    Plasmid
    #192738
    Purpose
    Gateway entry vector encoding zebrafish chodl
    Depositor
    Insert
    chodl (zgc:110088 Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    S192I
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 461 - 480 of 3499 results