We narrowed to 161,600 results for: addgene

1. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

   Plasmid

   #175571

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

   Plasmid

   #175572

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. eIF2a 1

   Plasmid

   #21807

   Depositor

   David Ron

   Insert

   eIF2a (EIF2S1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX551-CMV-SpCas9

   Plasmid

   #107024

   Purpose

   AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab

   Depositor

   Alex Hewitt

   Insert

   SpCas9
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. eIF2a 2

   Plasmid

   #21808

   Depositor

   David Ron

   Insert

   eIF2a (EIF2S1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S52A

   Promoter

   Available since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGFP_1

   Plasmid

   #238192

   Purpose

   Empty lentiviral vector for expressing protein sequences fused to GFP, under a TRE3GV promoter

   Depositor

   Feng Zhang

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP and SV40

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. eIF2a 3

   Plasmid

   #21809

   Depositor

   David Ron

   Insert

   eIF2a (EIF2S1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S52D

   Promoter

   Available since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT2/SV-Venus-SIINFEKL

   Plasmid

   #111624

   Purpose

   Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies

   Depositor

   Jon Yewdell

   Insert

   Venus-LEQLE-SIINFEKL-TEW
   

   Use

   Tags

   LEQLE-SIINFEKL-TEW

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. USP2

   Plasmid

   #36894

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   USP2 (USP2 Human)
   

   Use

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

10. p-dCas9-VP64-Hygro

    Plasmid

    #104405

    Purpose

    transient expression of dCas9-VP64 fusion protein

    Depositor

    Stephan Beck

    Insert

    VP64
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKG141

    Plasmid

    #69759

    Purpose

    Retroviral expression of CENP-B

    Depositor

    Iain Cheeseman

    Insert

    CENP-B (CENPB Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pXR003_puroR

    Plasmid

    #219819

    Purpose

    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. USP15

    Plasmid

    #28282

    Depositor

    Cheryl Arrowsmith

    Insert

    USP15 (USP15 Human)
    

    Use

    Tags

    His tag with thrombin cleavage

    Expression

    Bacterial

    Mutation

    Contains dusp and Ubl domains (residues 6-223).

    Promoter

    Available since

    April 14, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

14. CMV-dCas9_puroR

    Plasmid

    #219825

    Purpose

    CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    CMV

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE-CMV-Rheb(D60K)-IRES-eGFP-W

    Plasmid

    #32522

    Depositor

    Mustafa Sahin

    Insert

    dominant negative Rheb (D60K) (Rheb Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D60K to make dominant negative

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pBABE puro human RXR alpha

    Plasmid

    #11441

    Depositor

    Ronald Kahn

    Insert

    RXR alpha (RXRA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pBabe hygro human RXR alpha

    Plasmid

    #11440

    Depositor

    Ronald Kahn

    Insert

    RXR alpha (RXRA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

18. SLX4

    Plasmid

    #73242

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SLX4 (SLX4 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

    Plasmid

    #191529

    Purpose

    Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    H2BK_mNG_hSLBP(18-108)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mNeonGreen (mNG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lentiviral sgRNA human CD19

    Plasmid

    #155289

    Purpose

    Lentiviral expression of sgRNA against human CD19

    Depositor

    Martin Carroll

    Insert

    CD19 (CD19 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge