Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,468 results for: App

Clear keywords
Showing: 1151 - 1200 of 9468 results

  5. pJK598

    Plasmid
    #72523
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72523-plasmid-map-sequence-id-132608

    Enlarge

  6. pJK560

    Plasmid
    #71585
    Purpose
    Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with Arg232>His mutant (YfdE-R232H)
    Depositor
    T. J. Kappock
    Article
    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:
    Insert
    acetyl-CoA:oxalate CoA-transferase (yfdE )
    Expression
    Bacterial
    Mutation
    changed arginine-232 to histidine
    Promoter
    T7 promoter
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    71585-plasmid-map-sequence-id-130921

    Enlarge

  7. pJK504

    Plasmid
    #71584
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC) with C-terminal His6 tag and Cys357>Tyr mutant (AarCH6-C357Y)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)
    Insert
    succinyl-CoA:acetate CoA-transferase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes cysteine-357 to tyrosine
    Promoter
    T7
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71584-plasmid-map-sequence-id-131755

    Enlarge

  8. pJK612

    Plasmid
    #72533
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Expression
    Bacterial
    Mutation
    residues 1-69 only; C-terminal domain removed and…
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72533-plasmid-map-sequence-id-132621

    Enlarge

  9. pJK613

    Plasmid
    #72534
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    His6
    Expression
    Bacterial
    Mutation
    residues 1-69 only; C-terminal domain removed and…
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72534-plasmid-map-sequence-id-132623

    Enlarge

  10. pJK619

    Plasmid
    #72536
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72536-plasmid-map-sequence-id-132627

    Enlarge

  16. BiP-mGFP

    Plasmid
    #62231
    Purpose
    Encodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.
    Depositor
    Erik Snapp
    Article
    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2
    Insert
    hamster BiP-mGFP-KDEL
    Tags
    mGFP
    Expression
    Mammalian
    Promoter
    cmv
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    62231-plasmid-map-sequence-id-314151

    Enlarge

  17. Galt1-HT7-mGold

    Plasmid
    #230027
    Purpose
    Expressing fluorescent HaloTag7 fusion protein in mammalian cells with localization in the Golgi apparatus.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Galt1-HaloTag7-mGold (B4GALT1 Synthetic, Human)
    Tags
    HaloTag,mGold
    Expression
    Mammalian
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230027-plasmid-map-sequence-id-458169

    Enlarge

  19. Galt1-Grx1-roGFP1-iE

    Plasmid
    #230029
    Purpose
    Expressing Grx1-roGFP1-iE fluorescent fusion protein in mammalian cells with localization in the Golgi apparatus.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Galt1-Grx1-roGFP1-iE (B4GALT1 Synthetic, Human)
    Expression
    Mammalian
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230029-plasmid-map-sequence-id-458346

    Enlarge

  20. Galt1-sCGrx1p-HA

    Plasmid
    #230028
    Purpose
    Expressing sCGrx1p protein fused to HA tag in mammalian cells with localization in the Golgi apparatus.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Galt1-sCGrx1p-HA (B4GALT1 Synthetic, Human)
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230028-plasmid-map-sequence-id-458168

    Enlarge

  21. pRAM28

    Plasmid
    #234314
    Purpose
    pColE1 origin Cat-RNA U1376, Multiplexing ribozyme splicing reactions for the purpose of comparing host range of origins of replications
    Depositor
    James Chappell
    Article
    Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103
    Insert
    U1367 Targeting RAM, Barcode 2
    Use
    Synthetic Biology
    Promoter
    P. CymRC
    Available Since
    March 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234314-plasmid-map-sequence-id-466009

    Enlarge

  26. pKEK3238

    Plasmid
    #234092
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfCherry2
    Expression
    Bacterial
    Mutation
    E117Q/T127I/G219A
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234092-plasmid-map-sequence-id-467988

    Enlarge

  27. pKEK3233

    Plasmid
    #234105
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    YukonOFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234105-plasmid-map-sequence-id-464918

    Enlarge

  31. CyTERM-mGFP

    Plasmid
    #62237
    Purpose
    ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.
    Depositor
    Erik Snapp
    Article
    Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1
    Insert
    CyTERM-mGFP
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    signal anchor of rabbit cytochrome p450 and monom…
    Promoter
    CMV
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    62237-plasmid-map-sequence-id-320621

    Enlarge

  32. pKEK3395

    Plasmid
    #234079
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    YukonOFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234079-plasmid-map-sequence-id-464930

    Enlarge

  33. pKEK3228

    Plasmid
    #234090
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    aeBlue
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234090-plasmid-map-sequence-id-467993

    Enlarge

  34. pDE43-MCK

    Plasmid
    #49523
    Purpose
    Kanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning system
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49523-plasmid-map-sequence-id-73915

    Enlarge

  41. psecBFP2

    Plasmid
    #62230
    Purpose
    encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.
    Depositor
    Erik Snapp
    Article
    Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;
    Insert
    secBFP2
    Tags
    secbfp2
    Expression
    Mammalian
    Promoter
    cmv
    Available Since
    July 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  44. pJK511

    Plasmid
    #71591
    Purpose
    Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)
    Depositor
    T. J. Kappock
    Article
    Murphy et al Acta Crystallogr F Struct Biol Commun. 2
    Insert
    citrate synthase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes histidine-222 to glutamine
    Promoter
    T7 promoter
    Available Since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71591-plasmid-map-sequence-id-130911

    Enlarge

  45. pKEK3399

    Plasmid
    #234083
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    DasherGFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    234083-plasmid-map-sequence-id-477211

    Enlarge

  46. pKEK3225

    Plasmid
    #234087
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    DasherGFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    234087-plasmid-map-sequence-id-471771

    Enlarge

  47. pKEK3236

    Plasmid
    #234108
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfCherry2
    Expression
    Bacterial
    Mutation
    E117Q/T127I/G219A
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234108-plasmid-map-sequence-id-467997

    Enlarge

  48. pKEK3221

    Plasmid
    #234100
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfcherry 2
    Expression
    Bacterial
    Mutation
    E117Q/T127I/G219A
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234100-plasmid-map-sequence-id-468004

    Enlarge

  49. pKEK3397

    Plasmid
    #234081
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    FuGFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234081-plasmid-map-sequence-id-468005

    Enlarge

  50. pKEK3224

    Plasmid
    #234086
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    FuGFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234086-plasmid-map-sequence-id-468001

    Enlarge

Showing: 1151 - 1200 of 9468 results