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We narrowed to 4,045 results for: golden gate

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Showing: 1151 - 1200 of 4045 results

  1. C32m

    Plasmid
    #120967
    Purpose
    MoClo golden gate assembly CD part for CinR (Quorum-sensing receptor for 3OC14). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohCD carb CinR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120967-plasmid-map-sequence-id-236380

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  2. C36m

    Plasmid
    #120970
    Purpose
    MoClo golden gate assembly CD part for RhlI (quorum-sensing synthase for 3OC14). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohCD carb RhlI
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120970-plasmid-map-sequence-id-245423

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  3. C40m

    Plasmid
    #120973
    Purpose
    MoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Blank (Random 50bp sequence)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120973-plasmid-map-sequence-id-236027

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  4. C59m

    Plasmid
    #120976
    Purpose
    MoClo golden gate assembly CD part for mScarlet (RFP variant; see https://www.fpbase.org/protein/mscarlet/). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mScarlet
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120976-plasmid-map-sequence-id-236030

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  5. C13m

    Plasmid
    #120960
    Purpose
    MoClo golden gate assembly CD part for Cerulean (modified CFP; variant of https://www.fpbase.org/protein/cerulean/) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Cerulean
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120960-plasmid-map-sequence-id-245422

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  6. P1m

    Plasmid
    #120934
    Purpose
    MoClo golden gate assembly AB part plasmid for J23101 (strength 1791x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson); AB overhangs.
    Depositor
    Richard Murray
    Insert
    J23101
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120934-plasmid-map-sequence-id-235942

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  7. pICSL11086_GG_Wheat_live_Cas9_rev

    Plasmid
    #126882
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    126882-plasmid-map-sequence-id-249649

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  8. pAV10.F6.loxP

    Plasmid
    #63201
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Oct. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    63201-plasmid-map-sequence-id-267056

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  9. pECNUS4_MP

    Plasmid
    #228358
    Purpose
    ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    228358-plasmid-map-sequence-id-455680

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  11. pAV10.F3.loxP

    Plasmid
    #63200
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63200-plasmid-map-sequence-id-104877

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  12. pAV10.K6.loxP

    Plasmid
    #63203
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63203-plasmid-map-sequence-id-104900

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  13. pAV10.HO5.loxP

    Plasmid
    #63206
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63206-plasmid-map-sequence-id-104906

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  14. P18m

    Plasmid
    #120941
    Purpose
    MoClo golden gate assembly AB part for T7 promoter (strong, T7 polymerase-dependent constitutive promoter; use with C95m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120941-plasmid-map-sequence-id-235947

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  15. C31m

    Plasmid
    #120966
    Purpose
    MoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 10.1007/s00438-006-0129-5. Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    bxb1 integrase
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120966-plasmid-map-sequence-id-236888

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  16. P9m

    Plasmid
    #120937
    Purpose
    MoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Pr-OR1-OR2
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120937-plasmid-map-sequence-id-247706

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  17. pNDMS291

    Plasmid
    #248098
    Purpose
    sfCFP fluorescent reporter for synthetic Rhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    sfCFP
    Expression
    Bacterial
    Promoter
    Rlv_PsnifH
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248098-plasmid-map-sequence-id-495377

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  18. pNDMS290

    Plasmid
    #248097
    Purpose
    sfYFP fluorescent reporter for synthetic Rhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    sfYFP
    Expression
    Bacterial
    Promoter
    Rlv_PsnifH
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248097-plasmid-map-sequence-id-495376

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  19. pNDMS237

    Plasmid
    #248095
    Purpose
    sfYFP fluorescent reporter for synthetic Mesorhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    sfYFP
    Expression
    Bacterial
    Promoter
    Meso_PsnifH
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248095-plasmid-map-sequence-id-495374

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  20. pNDMS238

    Plasmid
    #248094
    Purpose
    sfCFP fluorescent reporter for synthetic Mesorhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    sfCFP
    Expression
    Bacterial
    Promoter
    Meso_PsnifH
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248094-plasmid-map-sequence-id-495373

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  21. pDest_PANCEd_AraC_sd2_R2_C1

    Plasmid
    #242149
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE variant cassette
    Depositor
    Dominik Niopek
    Insert
    AraC R2
    Expression
    Bacterial
    Mutation
    E3K, R146L, A152T, R251L
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242149-plasmid-map-sequence-id-484659

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  22. pKS1614

    Plasmid
    #239545
    Purpose
    Express truncated VoGES, Idi1, and Erg20WW for synthesis of geraniol in the peroxisome with Venus as peroxisomal marker
    Depositor
    John Dueber
    Insert
    tVoGES-ePTS1
    Expression
    Yeast
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239545-plasmid-map-sequence-id-479032

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  23. pKS1429

    Plasmid
    #239514
    Purpose
    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239514-plasmid-map-sequence-id-478375

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  24. pKS1173

    Plasmid
    #239507
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 5 high affinity SH3 peptides
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239507-plasmid-map-sequence-id-478369

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  25. pKS1334

    Plasmid
    #239508
    Purpose
    Express CDT2 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    NcCDT2-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    239508-plasmid-map-sequence-id-477192

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  26. pKS1140

    Plasmid
    #239501
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 2 high affinity SH3 peptides
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239501-plasmid-map-sequence-id-475913

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  27. pKS1577

    Plasmid
    #239525
    Purpose
    Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    ScVba2p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239525-plasmid-map-sequence-id-475912

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  28. pKS1141

    Plasmid
    #239502
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 5 high affinity SH3 peptides
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239502-plasmid-map-sequence-id-475904

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  29. pKS1461

    Plasmid
    #239515
    Purpose
    Express the lower portion of melvaonate pathway enzymes (Erg12p, Erg8p, and Erg19p) in the peroxisome; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    ScErg12p-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239515-plasmid-map-sequence-id-475897

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  30. pKS1172

    Plasmid
    #239506
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 2 high affinity SH3 peptides
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239506-plasmid-map-sequence-id-475884

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  31. pKS1428

    Plasmid
    #239513
    Purpose
    Express UTR1 membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    AtUTR1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239513-plasmid-map-sequence-id-475875

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  32. pVL1280

    Plasmid
    #239539
    Purpose
    Express CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    NcCDT1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239539-plasmid-map-sequence-id-475864

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  33. pAIP4_2RH3

    Plasmid
    #234219
    Purpose
    Heterologous protein expression of protein virC2 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2RH3 (virC2 Agrobacterium tumefaciens)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234219-plasmid-map-sequence-id-464184

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  34. pAIP4_5Y33

    Plasmid
    #234204
    Purpose
    Heterologous protein expression of alginate lyase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5Y33
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234204-plasmid-map-sequence-id-464169

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  35. pAIP4_4REH

    Plasmid
    #234195
    Purpose
    Heterologous protein expression of major latex-like protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4REH
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234195-plasmid-map-sequence-id-464160

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  36. pAIP4_4ZHW

    Plasmid
    #234158
    Purpose
    Heterologous protein expression of YfiB in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4ZHW (yfiB Pseudomonas aeruginosa PAO1)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234158-plasmid-map-sequence-id-464124

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  37. pAIP4_1XSZ

    Plasmid
    #234151
    Purpose
    Heterologous protein expression of RalF in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1XSZ (ralF Legionella pneumophila)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234151-plasmid-map-sequence-id-464117

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  38. pAIP4_2D5J

    Plasmid
    #234143
    Purpose
    Heterologous protein expression of unsaturated glucuronyl hydrolase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2D5J
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234143-plasmid-map-sequence-id-464109

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  39. L1B39 - pMUTE-Bxb1-tUBQ10

    Plasmid
    #219704
    Purpose
    Level 1 pMUTE-Bxb1-tUBQ10, for stomata specific expression of Bxb1 integrase
    Depositor
    Jennifer Nemhauser
    Insert
    pMUTE:Bxb1:tUBQ10
    Expression
    Plant
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219704-plasmid-map-sequence-id-435734

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  40. PROM_10

    Plasmid
    #201301
    Purpose
    D10: Promoter of TraesCS3D02G517600 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D10: Promoter of TraesCS3D02G517600
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    201301-plasmid-map-sequence-id-410981

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  41. pLM001

    Plasmid
    #201311
    Purpose
    AvrSr50 (with stop codon) as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr50
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    201311-plasmid-map-sequence-id-410106

    Enlarge

  42. pLM002

    Plasmid
    #201312
    Purpose
    AvrSr50 (no stop codon) as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr50
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    201312-plasmid-map-sequence-id-410095

    Enlarge

  43. PROM_3

    Plasmid
    #201296
    Purpose
    D3: Promoter of TraesCS3A02G510700 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D3: Promoter of TraesCS3A02G510700
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201296-plasmid-map-sequence-id-409521

    Enlarge

  44. PROM_12

    Plasmid
    #201303
    Purpose
    D12: Promoter of TraesCS4D02G131100 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D12: Promoter of TraesCS4D02G131100
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201303-plasmid-map-sequence-id-409517

    Enlarge

  45. PROM_4

    Plasmid
    #201297
    Purpose
    D4: Promoter of TraesCS3B02G552700 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D4: Promoter of TraesCS3B02G552700
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201297-plasmid-map-sequence-id-409516

    Enlarge

  46. PROM_6

    Plasmid
    #201299
    Purpose
    D6: Promoter of TraesCS3B02G578800 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D6: Promoter of TraesCS3B02G578800
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201299-plasmid-map-sequence-id-409515

    Enlarge

  47. PROM_5

    Plasmid
    #201298
    Purpose
    D5: Promoter of TraesCS3B02G553900 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D5: Promoter of TraesCS3B02G553900
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201298-plasmid-map-sequence-id-409513

    Enlarge

  48. PROM_1

    Plasmid
    #201294
    Purpose
    D1: Promoter of TraesCS1A02G332800 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D1: Promoter of TraesCS1A02G332800
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201294-plasmid-map-sequence-id-409512

    Enlarge

  49. PROM_7

    Plasmid
    #201300
    Purpose
    D7: Promoter of TraesCS3D02G316100 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D7: Promoter of TraesCS3D02G316100
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201300-plasmid-map-sequence-id-409511

    Enlarge

  50. PROM_2

    Plasmid
    #201295
    Purpose
    D2: Promoter of TraesCS2B02G533000 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D2: Promoter of TraesCS2B02G533000
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201295-plasmid-map-sequence-id-409509

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