We narrowed to 833 results for: Anti-CRISPR

1. pM215A

   Plasmid

   #173217

   Purpose

   Expresses ScFV-GCN4-sFGFP10M2-GB1

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   ScFV-GCN4-sfGFP10M2-GB1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLR01_pXL001_IL13CAR

   Plasmid

   #131086

   Purpose

   Overexpression of TQM-IL13-CAR

   Depositor

   Xiaojun Lian

   Insert

   TQM-IL13-CAR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. scFv-sfGFP-DNMT3A1

   Plasmid

   #102278

   Purpose

   The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

   Depositor

   Margaret Goodell

   Article

   Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

   Insert

   DNMT3A1 (DNMT3A Human)
   

   Use

   Lentiviral

   Tags

   scFV-sfGFP

   Expression

   Mammalian

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL7 lenti-dcas9-3xflag-blast

   Plasmid

   #112133

   Purpose

   lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   dCas9(D10A, N863A)-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A mutants in Cas9

   Promoter

   EF1a

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL8 lenti-D10Acas9-3xflag-blast

   Plasmid

   #112134

   Purpose

   lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   D10Acas9-3xflag-blast
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A mutant in Cas9

   Promoter

   EF1a

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLL4 sgRNA(MS2)-MS2-hA3a

   Plasmid

   #112130

   Purpose

   empty sgRNA cloning vector with MS2-humanA3a

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   human Apobec3a (APOBEC3A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. A3Ai-Cas9n-UGI-NLS

   Plasmid

   #109425

   Purpose

   Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Insertion of an L1 intron into the coding sequenc…

   Promoter

   CMV

   Available Since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL6 sgRNA(MS2)-MS2-hA3a D131E

   Plasmid

   #112132

   Purpose

   empty sgRNA cloning vector with MS2-humanA3a D131E

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   human Apobec3a D131E mutant (APOBEC3A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D131E mutant in hA3a

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL5 sgRNA(MS2)-MS2-hA3a D131A

   Plasmid

   #112131

   Purpose

   empty sgRNA cloning vector with MS2-humanA3a D131A

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   human Apobec3a D131A mutant (APOBEC3A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D131A mutant in hA3a

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHybr_r2a>m2a(silent)-srt-his

    Plasmid

    #124807

    Purpose

    HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Murine IgG2a (Fc silent)
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial and Mammalian

    Mutation

    L234A/L235A/N297A

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHybr_r2a>Fab-srt-his

    Plasmid

    #124810

    Purpose

    HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    rat IgG2a Hinge-G4S-Sortag-Histag
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial

    Promoter

    pUC

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHisIHFαIHFβ

    Plasmid

    #149384

    Purpose

    Expression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbeta

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Inserts

    Pseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
    Pseudomonas aeruginosa PA14 IHFbeta
    

    Tags

    6xHis tag and HRV3C cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pStrepIHFb

    Plasmid

    #149385

    Purpose

    Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
    

    Tags

    HRV3C cleavage site and StrepII-tag with

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PROM5_MpU6

    Plasmid

    #136120

    Purpose

    Mp U6 promoter (type III RNA polymerase promoter) to drive expression of gRNA for CRISPR/Cas9

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM5_MpU6
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-TetON-dual-SunTag-Hygro

    Plasmid

    #121119

    Purpose

    Endogenous gene activation with the SunTag CRISPR activation system

    Depositor

    Pablo Navarro

    Article

    Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

    Insert

    dCas9-GCN4x10_scFv-VP64
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Tet-ON

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS088

    Plasmid

    #211502

    Purpose

    AAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Type

    Empty backbone

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS006

    Plasmid

    #211506

    Purpose

    AAV genome backbone with a CROP-seq-like design for indirect capture of gRNA sequences

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Type

    Empty backbone

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115214

    Purpose

    Expresses PauCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PauCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDA_174_neoR

    Plasmid

    #228935

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti_ABE8e-SpRY-P2A-BFP_HygroR

    Plasmid

    #213009

    Purpose

    A lentiviral vector used to create stable cell lines expressing the ABE8e-SpRY base editor and BFP

    Depositor

    Richard Sherwood

    Article

    Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

    Insert

    ABE8e-SpRY-D10A
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A nickase variant of SpRY

    Promoter

    Ef-1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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