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Plasmid#50908PurposeExpresses human NKCC1 C723S C724V W732C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationC723S C724V W732C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V I725C (NT538)
Plasmid#50903PurposeExpresses human NKCC1 C723S C724V I725C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationC722S C723V I724C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V V726C (NT539)
Plasmid#50904PurposeExpresses human NKCC1 C723S C724V V726C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationC723S C724V V726C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 G719C C723S C724V (NT533)
Plasmid#50898PurposeExpresses human NKCC1 G719C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationG719C C723S C724V in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TetO-fSNAPc
Plasmid#106986Purposeopen reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sitesDepositorInsertfSNAP
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/FLAG-hPMR1-Tev-Bio
Plasmid#80125PurposeExpresses human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tagsDepositorInsertPXDNL-003 (PXDNL Human)
UseTagsFLAG and biotinylationExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)
Plasmid#49060PurposeExpresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequenceDepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFP (chloride-sensitive)ExpressionMammalianMutationCl-sensitive YFP (EYFP/ V163S A206K) in hNKCC1 in…PromoterCMVAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA HA HP1 beta
Plasmid#24079DepositorInsertHP1 beta (CBX1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro largeTcDNA
Plasmid#14088DepositorInsertLarge T antigen (SV40gp6 simian virus)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Myc-DNMT3L
Plasmid#35523DepositorInsertDNMT3L (DNMT3L Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 3xF-GAR1
Plasmid#126873PurposeExpresses 3xFlag GAR1DepositorInsertGAR1 (GAR1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ149-pcDNA3-BhCas12b_v4
Plasmid#122446PurposeExpresses BhCas12b_v4 in mammalian cellsDepositorInsertCMV-NLS-BhCas12b_v4-NLS-3xHA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Flag-XPA
Plasmid#22895DepositorInsertXPA (XPA Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pZ143-pcDNA3-BvCas12b
Plasmid#122445PurposeExpresses BvCas12b in mammalian cellsDepositorInsertCMV-NLS-BvCas12b-NLS-3xHA
UseTags3XHAExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
TLS 5:hTLS-CHOP.pCDNA1
Plasmid#21831DepositorInsertTLS-CHOP (FUS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4 rat NZAP
Plasmid#45908DepositorInsertZc3hav1 zinc finger CCCH type, antiviral 1 (Zc3hav1 Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4 HisMax-V5-GFP-RRBP1
Plasmid#92150PurposeExpresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.DepositorInsertRRBP1 (RRBP1 Human)
UseTagsCalreticulin signal peptide, EGFP (A207K mutation…ExpressionMammalianMutationIn RRBP1: N951S and T1329L. E1279 is also missing.PromoterCMVAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-CasE-p300-Flag
Plasmid#106275PurposeEncodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoterDepositorInsertEcoCasE-p300
UseCRISPRTagsE tag, NLS and FlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2-T92Q-sfGFP
Plasmid#145178PurposeMammalian expression plasmid for sACE2-sfGFP glycosylation mutant T92QDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-F-Med26-K74A K75A
Plasmid#49253PurposeMammalian expression of flag-tagged human Med26-K74A K75ADepositorInsertMed 26 (MED26 Human)
UseTagsHAExpressionMammalianMutationK74A K75APromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only