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Showing: 461 - 480 of 40799 results

  3. pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid
    #230058
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Use
    Tags
    6X HIS, HRV 3C PreScission protease, HaloTag, and…
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin (CAG)
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230058-plasmid-map-sequence-id-460119

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  4. pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid
    #230059
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA2 single chain variable fragment (scFv)
    Use
    Tags
    6X HIS, HRV 3C PreScission protease, HaloTag, and…
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin (CAG)
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230059-plasmid-map-sequence-id-460118

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  8. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  9. Anti-MMP9 matrix metalloproteinase-9 precursor [L51/82R]

    Plasmid
    #149455
    Purpose
    Mammalian Expression Plasmid of anti-MMP9 matrix metalloproteinase-9 precursor (Rat). Derived from hybridoma L51/82.
    Depositor
    James Trimmer
    Article
    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1
    Insert
    anti-MMP9 matrix metalloproteinase-9 precursor (Rattus norvegicus) recombinant mouse monoclonal antibody (Mmp9 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    149455-plasmid-map-sequence-id-302040

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  14. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail

    Plasmid
    #60392
    Purpose
    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoter
    Depositor
    Ron Vale
    Article
    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
    Insert
    TUB1 (TUB1 Budding Yeast, Synthetic)
    Use
    Tags
    Expression
    Yeast
    Mutation
    internal 6xHis (see supplement figure 4), amino a…
    Promoter
    GAL
    Available since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. Anti-LRRK2/Dardarin kinase, C-terminus [N241A/58R]

    Plasmid
    #220411
    Purpose
    Mammalian Expression Plasmid of anti-LRRK2/Dardarin kinase, C-terminus (Human) IgG2a R-mAb. Derived from hybridoma N241A/58
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-LRRK2/Dardarin kinase, C-terminus (Homo sapiens) recombinant mouse monoclonal antibody. (LRRK2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    220411-plasmid-map-sequence-id-435564

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Showing: 461 - 480 of 40799 results