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LAMP-ReNL_pcDNA3

Plasmid

#89532

Purpose

Red color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

LAMP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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P305-FBP_4

Plasmid

#139695

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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CeNL-Clathrin_pEGFP

Plasmid

#89540

Purpose

Cyan color variant of bright luminescent protein, fused to clathrin light chain

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Use

Tags

Clathrin light chain

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET-CV-Ykt6

Plasmid

#168030

Purpose

Expresses eCFP-Ykt6(Q131-K190)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-Ykt6(Q131-K190)-mVenus

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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APT3-VAMP4

Plasmid

#168004

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP4(I75-L91)-T3'PAP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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APT3-PTEN (PTEN high)

Plasmid

#168006

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

PTEN-T3'PAP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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LOTUS-V/pcDNA3

Plasmid

#87127

Purpose

Bioluminescent and ratiometric voltage indicator

Depositor

Takeharu Nagai

Article

Inagaki et al Sci Rep. 2017 Feb 13;7:42398. doi: 10.10

Insert

LOTUS-V

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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LAMP-OeNL_pcDNA3

Plasmid

#89525

Purpose

Orange color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

LAMP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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APneg-VAMP1a

Plasmid

#168007

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP1(T29-K89)-T7PAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(Ca2+)_11/pcDNA3

Plasmid

#51987

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-4GS

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern(Ca2+)_150/pcDNA3

Plasmid

#51984

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=150nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-2G

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern-mitochondria/pcDNA3

Plasmid

#51973

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to mitochondria

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

CoxVIIIx2

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

March 28, 2014

Availability

Academic Institutions and Nonprofits only
P303-FBP_2

Plasmid

#139693

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CeNL(Ca2+)_1.2μ-pcDNA3

Plasmid

#111926

Purpose

Cyan color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_1.2μ

Use

Tags

Expression

Mammalian

Mutation

E67D, E104D, D133E at CaM

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(Ca2+)_300/pcDNA3

Plasmid

#51983

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-3GS

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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OeNL(Ca2+)_18μ-pcDNA3

Plasmid

#111928

Purpose

Orange color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

OeNL(Ca2+)_18μ

Use

Tags

Expression

Mammalian

Mutation

E67D, E104D, D133E at CaM

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pTPI1-EMC1-GFP-APEX2-URA3

Plasmid

#184776

Purpose

Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

APEX2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_19μ-pcDNA3

Plasmid

#111927

Purpose

Cyan color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_19μ

Use

Tags

Expression

Mammalian

Mutation

E67D, F92W, E104D, D133E at CaM

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Nano-lantern(Ca2+)_600/pcDNA3

Plasmid

#51982

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-2G

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
APneg-VAMP1d

Plasmid

#168010

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP1(T29-K89)-T7PAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge