We narrowed to 8,446 results for: Ott

1. pRSET-pr-mKikGR1 (mKikGR1-V69T)

   Plasmid

   #99227

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mKikGR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   monomeric variant with mutation V69T (Eos notatio…

   Promoter

   T7

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmScarlet-H_Giantin_C1

   Plasmid

   #85049

   Purpose

   In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Giantin (GOLGB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa 3131-3259

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

   Plasmid

   #163169

   Purpose

   Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   Non-targeting
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCKO_PSMD1_sgRNA_5

   Plasmid

   #74181

   Purpose

   lentiviral vector expressing sgRNA targeting PSMD1

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   PSMD1 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. myc-Atg14 S29D

   Plasmid

   #86757

   Purpose

   N-terminal myc-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

   Insert

   atg14 (ATG14 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S29D

   Promoter

   CMV

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSRW1JZ

   Plasmid

   #129528

   Purpose

   C. elegans codon-optimized FlincG3 (GFP-based cGMP sensor) expressed in C. elegans ASE neuron pair

   Depositor

   Noelle L'Etoile

   Article

   Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

   Insert

   FlincG3
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1

   Plasmid

   #121831

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/LSD1 (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. UbG76V-eGFP-V5His_15aa

   Plasmid

   #23969

   Depositor

   Nico Dantuma

   Article

   Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

   Insert

   Ub-G76V-eGFP-V5HIS_15aa (UBB Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   G76V mutation in Ubiquitin. Ub fused to N-termin…

   Promoter

   Available since

   April 16, 2010

   Availability

   Academic Institutions and Nonprofits only

9. (p)odr-3::GFP::EGL-4

   Plasmid

   #100897

   Purpose

   GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.

   Depositor

   Noelle L'Etoile

   Article

   Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10

   Insert

   56-2741: odr-3 promoter
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   odr-3

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNCST-AvicFP1

    Plasmid

    #129504

    Purpose

    Expresses AvicFP1 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HtrA2-EGFP S306A

    Plasmid

    #14122

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S306A

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

    Plasmid

    #163698

    Purpose

    AAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortex

    Depositor

    Gina Turrigiano

    Article

    Trojanowski et al Neuron. 2021 Feb 17;109(4):663-676.e5. d

    Insert

    CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII fragment

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p6348 MSCV-CMV-Flag-HA-Brd4 1047-1362

    Plasmid

    #31354

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    Brd4 1047-1362 (BRD4 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEMS1996

    Plasmid

    #49126

    Purpose

    AAV plasmid with UGT8 (Ple267) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple267-iCre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UGT8

    Available since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pEMS1977

    Plasmid

    #49109

    Purpose

    AAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple251-icre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    C8ORF46

    Available since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. myc-Atg14 S29A

    Plasmid

    #86756

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLCKO_PSMD1_sgRNA_1

    Plasmid

    #74180

    Purpose

    lentiviral vector expressing sgRNA targeting PSMD1

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    PSMD1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNCST-AausGFP

    Plasmid

    #129503

    Purpose

    Expresses AausGFP constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p6347 MSCV-CMV-Flag-HA-Brd4-444-722

    Plasmid

    #31353

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    Brd4 444-722 (BRD4 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3E-CDC42-DN

    Plasmid

    #109584

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative CDC42

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    CDC42 (cdc42 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    dominant negative

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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