We narrowed to 9,202 results for: sacs

1. pPK-351

   Plasmid

   #157921

   Purpose

   pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD

   Depositor

   Phillip Kyriakakis

   Article

   Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

   Inserts

   PIF3-MTAD
   IRES
   PhyB(1-621)-SV40NLS-Gal4DBD
   

   Use

   Synthetic Biology; Optogenetics

   Tags

   HA tag and SV40NLS

   Expression

   Mammalian

   Mutation

   pif3 1-523

   Promoter

   CMV

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc-HT-Dmp53KRKR

   Plasmid

   #85699

   Purpose

   expression of Drosophila p53 KRKR mutant in Drosophila cells

   Depositor

   Giannino Del Sal, Licio Collavin

   Article

   Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

   Insert

   Dmp53 (p53 Fly)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   K26R K302R

   Promoter

   Ac5

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

3. AB.pCCL.sin.cPPT.U6.miR-210-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46599

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-210 Decoy (MIR210, Mir210 Human, Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCfB3038(XII-1 MarkerFree)

   Plasmid

   #73278

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfB3040(XII-4 MarkerFree)

   Plasmid

   #73280

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTH399-SUP45-S30P

   Plasmid

   #29372

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   S30P mutant gene (corresponding to the sl23ts all…

   Promoter

   Available since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pTH375-SUP45-P174Q

   Plasmid

   #29383

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   P174Q mutant gene including genomic sequence 1432…

   Promoter

   Available since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pDUAL-P41nmt1-Nup124-GFP-MTn

   Plasmid

   #154365

   Purpose

   Yeast expression of Nup124

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   Nup124 (nup124 Fission Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   See depositor comments below

   Promoter

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Flag-Fos S374A

   Plasmid

   #118302

   Purpose

   Expression of mouse C-Fos

   Depositor

   Philip Stork

   Article

   Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

   Insert

   C-Fos S374A (Fos Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S374A

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS316-VTC4

    Plasmid

    #108691

    Purpose

    VTC4 sequence expressed in yeast plasmid

    Depositor

    Michael Downey

    Article

    Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

    Insert

    Vacuolar Transporter Chaperone 4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    VTC4 (native promoter)

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 908E

    Plasmid

    #160238

    Purpose

    TtgO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Ttg Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 908G

    Plasmid

    #160239

    Purpose

    PipO(3x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Pip Operator (3x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 908J

    Plasmid

    #160242

    Purpose

    CymO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Cym Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AB.pCCL.sin.cPPT.U6.miR-27b-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46603

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-27b Decoy (MIR27B, Mir27b Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCOG75-Sem1 Sc

    Plasmid

    #83233

    Purpose

    pCDF-His6-MBP-Sem1 Sc

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    SEM 1 (SEM1 Budding Yeast)
    

    Use

    Tags

    His-mbp

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NG0888 pCI-TPI-PTC48-HBB

    Plasmid

    #65802

    Purpose

    NMD reporter mRNA

    Depositor

    Niels Gehring

    Article

    Clerici et al Nucleic Acids Res. 2014 Feb;42(4):2673-8

    Insert

    TPI (TPI1 Human)
    

    Use

    Tags

    HBB (beta-globin 3'UTR for probe binding)

    Expression

    Mammalian

    Mutation

    premature stop codon at 48

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCfB3037(XI-5 MarkerFree)

    Plasmid

    #73277

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Cwc25-pRSETA

    Plasmid

    #61043

    Purpose

    Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.

    Depositor

    Nils Walter

    Article

    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

    Insert

    Cwc25
    

    Use

    Tags

    TEV site, Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pAG415-ADH1p-HA-rap1-K9R

    Plasmid

    #84531

    Purpose

    This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    K(43,61,69,117,240,246,527,582,651)R

    Promoter

    ADH1

    Available since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pAG415-GPDp-EGFP-rap1-K3NR

    Plasmid

    #84533

    Purpose

    This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    K(43,117,118)R

    Promoter

    GPD

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pAG415-GPDp-EGFP-rap1-K3CR

    Plasmid

    #84534

    Purpose

    This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    K(462,463,651)R

    Promoter

    GPD

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pAG415-GPDp-EGFP-rap1-K9R

    Plasmid

    #84535

    Purpose

    This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    K(43,61,69,117,240,246,527,582,651)R

    Promoter

    GPD

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pDONR221-rap1-K462-463-651R

    Plasmid

    #84564

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Tags

    Expression

    Mutation

    K462-463-651R

    Promoter

    none

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDONR221-rap1-K43-117-118R

    Plasmid

    #84565

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Tags

    Expression

    Mutation

    K43-117-118R

    Promoter

    none

    Available since

    Oct. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGS1212

    Plasmid

    #63785

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eGFP-Strep-tag II followed by URA3 selection marker
    

    Use

    E. coli cloning vector

    Tags

    eGFP-Strep-tag II

    Expression

    Mutation

    Promoter

    none

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRS424-TUB2 - human TUBB6 Cterminal tail

    Plasmid

    #60403

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

27. AB.pCCL.sin.cPPT.U6.miR-139-5p-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46588

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-139-5p Decoy (MIR139, Mir139 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

28. AB.pCCL.sin.cPPT.U6.miR-127-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46583

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-127 Decoy (MIR127, Mir127 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

29. AB.pCCL.sin.cPPT.U6.miR-130b-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46586

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-130b Decoy (MIR130B, Mir130b Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

30. AB.pCCL.sin.cPPT.U6.miR-100-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46580

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-100 Decoy (MIR100, Mir100 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only