We narrowed to 18,359 results for: met
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Plasmid#169191PurposeTo express nsp10-nsp16 fusion protein in E. coliDepositorInsert14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab SARS-CoV-2, Synthetic)
UseTags14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)
Plasmid#187844PurposeExpression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
UseTagsIg k-chain leader sequence, SNAPf-tag, and meGFPe…ExpressionMammalianMutationmeGFPe: asparagine 198 to aspartic acid and tyros…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]
Plasmid#84483PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.DepositorInsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]
Plasmid#128587PurposeAAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.DepositorInsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterEF1α (1.1 kb short version)Available SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)
Plasmid#192785PurposeExpression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
UseTagsIg k-chain leader sequence, SNAPf-tag, and mXFPeExpressionMammalianMutationmXFPe: tyrosine 66 to phenylalanine, asparagine 1…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
GEST-COMP-blac-flag-his
Plasmid#111015PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertgamete egress and sporozoite traversal protein (GEST) (PF3D7_1449000 )
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable SinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)
Plasmid#192704PurposeExpression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
UseTagsIg k-chain leader sequence, SNAPf-tag, and meGFPm…ExpressionMammalianMutationmeGFPm: gluatmic acid 142 to asparagine and histi…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)
Plasmid#192784PurposeExpression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
UseTagsIg k-chain leader sequence, SNAPf-tag, and mXFPmExpressionMammalianMutationmXFPm: tryptophan 66 to phenylalanine, gluatmic a…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF806-shABC1
Plasmid#186713PurposeExpresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.DepositorUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF806-shABC2
Plasmid#186714PurposeExpresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.DepositorUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-YFP
Plasmid#55781PurposeThis G protein alpha-s construct contains internal insertions of YFP and the EE epitope.DepositorInsertalpha-s-EE YFP (Gnas Rat)
UseTagsEYFP flanked by SGGGS on each side was inserted i…ExpressionMammalianMutationMet was substituted for Gln69 in EYFP (Clontech).…PromoterCMVAvailable SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGLU_113
Plasmid#225794PurposeRS421 library variant 13453DepositorInsert13453_[Tn5]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_95
Plasmid#225809PurposeRS421 library variant 13453DepositorInsert13453_[Himar]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_77
Plasmid#225779PurposeRS421 library variant 13453DepositorInsert13453_[abr]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRP935
Plasmid#159302PurposeYeast tagging vector to create gene fusion with yeast codon optimized mAmetrine under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT1K
Plasmid#105707PurposeBacterial expression plasmids for production of recombinant metallothionein 1kDepositorInsertMetallothionein 1k
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIAneo
Plasmid#52118Purposea bicistronic IRES-mAmetrine 1.1 containing vector for mammalian expression (CMV promoter)DepositorTypeEmpty backboneUseTagsIRES-mAmetrine 1.1ExpressionMammalianMutationPromoterCMVAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQFKan
Plasmid#32321DepositorInsertQF Kan
UseTagsExpressionMutationPromoterAvailable SinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX-CS-MjPiwi_G
Plasmid#146384PurposeBacterial Expression of MjPiwiDepositorInsertMjPiwi
UseTagsExpressionBacterialMutationone missense mutation C490A (H163N) compared to t…PromoterAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT1G
Plasmid#105704PurposeBacterial expression plasmids for production of recombinant metallothionein 1gDepositorInsertMetallothionein 1g (MT1G Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only