We narrowed to 13,263 results for: cDNA

1. pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)

   Plasmid

   #70669

   Purpose

   Human mutant of E2F1 lacking amino acids 284 to 359

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F1 lacking residues 284 to 359 (E2F1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   lacks amin acids 284 to 359

   Promoter

   cmv

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pcdna3 T7 Jip1b(282-707)(C605S, C631S, C643S and C696T)

   Plasmid

   #52134

   Purpose

   a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

   Insert

   JNK interacting protein-1b (JIP-1b)(282-707)(M1-5) (Mapk8ip1 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-281 and C605S, C631S, C643S …

   Promoter

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1 Flag YFP hNKCC1 C723S C724V A735C (NT549)

   Plasmid

   #50910

   Purpose

   Expresses human NKCC1 C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   C723S C724V A735C in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 Flag YFP hNKCC1 A720C C723S C724V (NT534)

   Plasmid

   #50899

   Purpose

   Expresses human NKCC1 A720C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   A720C C723S C724V in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 Flag YFP hNKCC1 L718C C723S C724V (NT532)

   Plasmid

   #50897

   Purpose

   Expresses human NKCC1 L718C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   L718C C723S C724V in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 Flag YFP hNKCC1 L722C C723S C724V (NT536)

   Plasmid

   #50901

   Purpose

   Expresses human NKCC1 L722C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   L722C C723S C724V in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 Flag YFP hNKCC1 S716C C723S C724V (NT530)

   Plasmid

   #50895

   Purpose

   Expresses human NKCC1 S716C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   S716C C723S C724V in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 Flag YFP hNKCC1 I721C C723S C724V (NT535)

   Plasmid

   #50900

   Purpose

   Expresses human NKCC1 I721C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   I721C C723S C724V in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 Flag YFP hNKCC1 C723S C724V V729C (NT543)

   Plasmid

   #50907

   Purpose

   Expresses human NKCC1 C723S C724V V729C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   C723S C724V V729C in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 Flag YFP hNKCC1 C723S C724V W732C (NT546)

    Plasmid

    #50908

    Purpose

    Expresses human NKCC1 C723S C724V W732C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V W732C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 Flag YFP hNKCC1 C723S C724V W733C (NT547)

    Plasmid

    #50909

    Purpose

    Expresses human NKCC1 C723S C724V W733C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V W733C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 Flag YFP hNKCC1 C723S C724V F728C (NT542)

    Plasmid

    #50906

    Purpose

    Expresses human NKCC1 C723S C724V F728C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V F728C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 Flag YFP hNKCC1 C723S C724V I725C (NT538)

    Plasmid

    #50903

    Purpose

    Expresses human NKCC1 C723S C724V I725C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C722S C723V I724C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 Flag YFP hNKCC1 C723S C724V V726C (NT539)

    Plasmid

    #50904

    Purpose

    Expresses human NKCC1 C723S C724V V726C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V V726C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 Flag YFP hNKCC1 C723S C724V M727C (NT540)

    Plasmid

    #50905

    Purpose

    Expresses human NKCC1 C723S C724V M727C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V M727C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 Flag YFP hNKCC1 G719C C723S C724V (NT533)

    Plasmid

    #50898

    Purpose

    Expresses human NKCC1 G719C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    G719C C723S C724V in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Kv7.4 (1-645, Δ368-523)/S536A/L536A/HA tag/pcDNA3.1

    Plasmid

    #111462

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523)/S536A/L540A, C643A

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Kv7.4 (1-645, Δ368-523)/S536D/L540D/HA tag/pcDNA3.1

    Plasmid

    #111497

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523)/S536D/L540D, C643A

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Myc-tagged delta N (dominant-negative form) human TCF4E pcDNA3

    Plasmid

    #32739

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    TCF4E (Tcf7l2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    The N-terminal deletion form (delta N) lacks amin…

    Promoter

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid

    #87829

    Purpose

    High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    MTS
    NLS
    PTS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

    Plasmid

    #169337

    Purpose

    Expression of the LT-Fucci(SCA) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Fucci(SCA) (GMNN, CDT1 Human)
    

    Use

    Tags

    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHS0810 pcDNA3.1(+) CMV-SV40 NLS-OgeuIscB-nucleoplasmin NLS-3xHA

    Plasmid

    #176540

    Purpose

    Mammalian expression of nuclear localized OgeuIscB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    OgeuIscB
    

    Use

    Tags

    Nucleoplasmin NLS-3xHA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI

    Plasmid

    #205162

    Purpose

    express scFv-GCN4-HAtag-msfGFP(V206K)-GBI

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    scFv-GCN4-HAtag-msfGFP(V206K)-GBI
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA

    Plasmid

    #176538

    Purpose

    Mammalian expression of nuclear localized AwaIscB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    AwaIscB
    

    Use

    Tags

    Nucleoplasmin NLS-3xHA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized

    Plasmid

    #166834

    Purpose

    Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    TEV-GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

    Plasmid

    #228400

    Purpose

    Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm

    Depositor

    Dirk Daelemans

    Article

    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

    Insert

    NES/PKI-mNeonGreen2x-NLS/FUS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3

    Plasmid

    #206093

    Purpose

    Cav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    E314A, E664A, E1370A, E1658A

    Promoter

    CMV

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid

    #182682

    Purpose

    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Used as a donor for endogenous NFL tagging via Targeted Knock-In with Two guides (TKIT) approach.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    NA

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid

    #182685

    Purpose

    Encodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and NA

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/S1421R/P1424Q/Y1426S)

    Plasmid

    #155010

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    E1418K/A1419R/S1421/P1424Q/Y1426S; insert codes f…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge