We narrowed to 30,121 results for: LIS

1. pX601‐mhCMV‐ABEmaxNGA‐C2‐ E53ogRNA

   Plasmid

   #187066

   Purpose

   Gp41-1 Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

   Depositor

   Renzhi Han

   Article

   Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

   Insert

   Gp41-1-ABEmaxNGA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mhCMV

   Available since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. A3A-PBE-ΔUGI

   Plasmid

   #119770

   Purpose

   Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-ΔUGI-NLS
   

   Use

   Tags

   NLS

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   maize Ubiquitin-1

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET SUMO-FL-syt1

   Plasmid

   #213628

   Purpose

   Bacterial expression of SUMO-tagged rat FL-syt1

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   FL-syt1 (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRibo-APEX2m

   Plasmid

   #176842

   Purpose

   Expression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-APEX2m
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-PIWI

   Plasmid

   #11591

   Depositor

   Edward Chan

   Article

   Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

   Insert

   Ago2 (AGO2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contain only aa482-869

   Promoter

   Available since

   June 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. NHA-TNGW1

   Plasmid

   #36437

   Depositor

   Edward Chan

   Article

   Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   NHA

   Expression

   Mammalian

   Mutation

   Full length (aa1-1962)

   Promoter

   Available since

   June 1, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

   Plasmid

   #132682

   Purpose

   AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

   Depositor

   Jonathan Long

   Article

   Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

   Insert

   Peptidase M20 domain containing 1 (Pm20d1 Mouse)
   

   Use

   AAV

   Tags

   6xHIS and Flag

   Expression

   Mutation

   Promoter

   Chicken b-actin

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-EF1A-LivePAR(R163A)-Hygro

   Plasmid

   #187609

   Purpose

   EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.

   Depositor

   Robert Sobol

   Article

   Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

   Insert

   LivePAR(R163A)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R163A mutation in the WWE Domain

   Promoter

   EF1A

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MSCVpic2blast-EGFP-FF2

   Plasmid

   #164923

   Purpose

   Expresses EGFP cDNA and FF2 shRNA in mammalian cells

   Depositor

   Ji Luo

   Article

   Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

   Inserts

   Enhanced green fluorescent protein
   Neuropeptide FF receptor 2 (NPFFR2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Geph CR3m shRNA

    Plasmid

    #121069

    Purpose

    Negative control shRNA for plasmid 121068: shRNA targeting the coding region of the gephyrin mRNA

    Depositor

    Angel de Blas

    Article

    Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

    Insert

    GPHN shRNA (Gphn Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pQCXIH-Flag-YAP-S381A

    Plasmid

    #33068

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S381A

    Promoter

    CMV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pSumo-His6- Sumo-AtTPL(1-209)

    Plasmid

    #177856

    Purpose

    Bacterial Expression of TPL

    Depositor

    Karsten Melcher

    Article

    Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

    Insert

    TPL (TPL Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-JNKAR1-NES

    Plasmid

    #84632

    Purpose

    Unimolecular, FRET-based JNK activity reporter; cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    ECFP-FHA1-JNKsubDD-Citrine-NES
    

    Use

    Tags

    6xHis, Citrine, ECFP, Nuclear export signal (NES)…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TL021_lenti_CMV:SEP-GluA1

    Plasmid

    #192515

    Purpose

    Expression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusion

    Depositor

    Adam Cohen

    Article

    Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

    Insert

    SEP-GluA1
    

    Use

    Lentiviral

    Tags

    super ecliptic phluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIVT/Smarcc1

    Plasmid

    #122145

    Purpose

    For making Smarcc1 RNA

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Smarcc1 (Smarcc1 Mouse)
    

    Use

    For in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR-QK1

    Plasmid

    #16183

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    Quaking (QKI Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET42b-A3A-PBE-ΔUGI

    Plasmid

    #119771

    Purpose

    To express the recombinant APOBEC3A in vitro

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS
    

    Use

    Tags

    6*HIS and NLS

    Expression

    Bacterial

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PlmdCas12e KRAB

    Plasmid

    #188506

    Purpose

    Expresses FLAG tagged PlmdCas12e KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-FLAG-KRAB-NLS

    Expression

    Mammalian

    Mutation

    D659A/E756A/D922A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-Cerulean-WW-NES

    Plasmid

    #84629

    Purpose

    Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    Cerulean-WW-NES
    

    Use

    Tags

    6xHis, Cerulean, Nuclear export signal (NES), T7 …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Luc-Pten-3'UTR

    Plasmid

    #64098

    Purpose

    Pten Luciferase reporter

    Depositor

    Lin He

    Article

    Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

    Insert

    Pten 3'UTR (PTEN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only