We narrowed to 13,291 results for: cDNA

1. pcDNA3-Flag-YOD1 C160S

   Plasmid

   #49785

   Purpose

   Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

   Depositor

   Christian Schlieker

   Article

   Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

   Insert

   YOD1 (YOD1 Human)
   

   Use

   Tags

   Flag-tag

   Expression

   Mutation

   C160S

   Promoter

   Available Since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1(+)-ExRai-AKAR-Kras

   Plasmid

   #118408

   Purpose

   Plasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR-Kras
   

   Use

   Tags

   C-terminal targeting sequence from Kras

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-DDB1 (1140H)

   Plasmid

   #174849

   Purpose

   Expression of DDB1

   Depositor

   Helen Morrison

   Insert

   DDB1 (DDB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5/FRT/TO GFP DNAJA4

   Plasmid

   #19494

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJA4 (DNAJA4 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-GFP-2AFP-KDEL

   Plasmid

   #154349

   Purpose

   Mammalian expression of 2AFP

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   2AFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.2/V5 hsa-mir-214

   Plasmid

   #26313

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   hsa-mir-214 (MIR214 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

7. GFP Cav2.2 HA D122A pcDNA3

   Plasmid

   #206120

   Purpose

   expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D122A

   Promoter

   CMV

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cav2.2 HA E1658A (EIVA) pcDNA3

   Plasmid

   #206114

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1658A

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cav2.1 EIVA (E1707A) pcDNA3

   Plasmid

   #206125

   Purpose

   expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1a (Cacna1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1707A

   Promoter

   CMV

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cav2.1 HA EIA (E320A) pcDNA3

    Plasmid

    #206124

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A

    Promoter

    CMV

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HA-REDD1 77-232 (#426)

    Plasmid

    #99921

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal deletion; encodes AA 77-232

    Promoter

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-TAT-1-delta2-26-FLAG

    Plasmid

    #35983

    Depositor

    Susana Valente

    Article

    Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

    Insert

    transactivating protein (tat )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    tat from HIV1 aa 2-26 deleted

    Promoter

    Available Since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-Lyn-sACt-mCherry

    Plasmid

    #138216

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    Lyn-sACt-mCherry (Adcy10 Rat)
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-deltaA1-Lock-VWF

    Plasmid

    #124824

    Purpose

    Expresses multimeric human VWF protein without A1 domain and Lock A2 domain. This protein has C-terminal c-Myc tag.

    Depositor

    Sriram Neelamegham

    Article

    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

    Insert

    VWF without A1 domain and Lock A2 domain (VWF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3 WT MYOC eGLuc2 FLAG

    Plasmid

    #192820

    Purpose

    Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag

    Depositor

    John Hulleman

    Article

    Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. do

    Insert

    myocilin (MYOC Human)
    

    Use

    Tags

    FLAG and enhanced Gaussia luciferase (M43I/M110I)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

    Plasmid

    #21800

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    PSMD14 (Psmd14 Mouse)
    

    Use

    Tags

    GST and TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-S586A-VLCAD-FLAG

    Plasmid

    #55824

    Purpose

    Expresses S586A VLCAD mutant in mammalian cells

    Depositor

    Yoshimi Homma

    Article

    Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

    Insert

    Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    changed Serine 586 toAlanine

    Promoter

    CMV

    Available Since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-zeo-hFJX1-myc

    Plasmid

    #46930

    Purpose

    expression of human FJX1-myc in mammalian cells

    Depositor

    Natasha Deane, R.D. Beauchamp

    Insert

    FJX1 (FJX1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

    Plasmid

    #181843

    Purpose

    GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of residues 12-112 in PKA RIα.

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

    Plasmid

    #194684

    Purpose

    CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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