We narrowed to 11,122 results for: ARIA

1. MAC-C Sars-CoV-2 NSP13_P77L

   Plasmid

   #194815

   Purpose

   Sars-CoV-2 NSP13_P77L in MAC-C destination vector

   Depositor

   Markku Varjosalo

   Article

   Varjosalo et al 10 May 2024, Research Square

   Insert

   NSP13_P77L (ORF1ab Sars-CoV-2)
   

   Use

   Tags

   MAC-C

   Expression

   Mammalian

   Mutation

   P77L

   Promoter

   Available since

   May 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. MAC-C Sars-CoV-2 NSP2_T85I

   Plasmid

   #194816

   Purpose

   Sars-CoV-2 NSP2_T85I in MAC-C destination vector

   Depositor

   Markku Varjosalo

   Article

   Varjosalo et al 10 May 2024, Research Square

   Insert

   NSP2_T85I (ORF1ab Sars-CoV-2)
   

   Use

   Tags

   MAC-C

   Expression

   Mammalian

   Mutation

   T85I

   Promoter

   Available since

   May 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. MAC-C Sars-CoV-2 NSP3_A890D

   Plasmid

   #194817

   Purpose

   Sars-CoV-2 NSP3_A890D in MAC-C destination vector

   Depositor

   Markku Varjosalo

   Article

   Varjosalo et al 10 May 2024, Research Square

   Insert

   NSP3_A890D (ORF1ab Sars-CoV-2)
   

   Use

   Tags

   MAC-C

   Expression

   Mammalian

   Mutation

   A890D

   Promoter

   Available since

   May 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. MAC-C Sars-CoV-2 NSP3_K977Q

   Plasmid

   #194818

   Purpose

   Sars-CoV-2 NSP3_K977Q in MAC-C destination vector

   Depositor

   Markku Varjosalo

   Article

   Varjosalo et al 10 May 2024, Research Square

   Insert

   NSP3_K977Q (ORF1ab Sars-CoV-2)
   

   Use

   Tags

   MAC-C

   Expression

   Mammalian

   Mutation

   K977Q

   Promoter

   Available since

   May 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCDNA3-SREBP-1-GFP (A317-318; A341; A1171)

   Plasmid

   #204199

   Purpose

   Mammalian expression of SREBP-1 (isoform 1)

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Use

   Tags

   3xHA, eGFP and T7x2

   Expression

   Mammalian

   Mutation

   Gly to Ala 317,318,341,1171

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-RVR-A3A-Y130F

   Plasmid

   #193658

   Purpose

   Expression of base editor RVR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   RVR-dLbCas12a-hA3A-Y130F, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-Y130F

   Promoter

   CMV

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-SKP1-K142R

   Plasmid

   #213423

   Purpose

   Expresses human SKP1 K142R mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

   Insert

   human Flag tagged SKP1-K142R (SKP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Flag-SKP1-97-101-5A

   Plasmid

   #213642

   Purpose

   Expresses human SKP1-97-101-5A mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

   Insert

   human Flag tagged SKP1--97-101-5A (SKP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-SKP1-K163R

   Plasmid

   #213424

   Purpose

   Expresses human SKP1 K163R mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

   Insert

   human Flag tagged SKP1-K163R (SKP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-enCas12a-A3A-N57G

    Plasmid

    #193651

    Purpose

    Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLb-enCas12a-hA3A-N57G, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-RVR-A3A-N57G

    Plasmid

    #193657

    Purpose

    Expression of base editor RVR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    RVR-dLbCas12a-hA3A-N57G, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-N57G

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6-AN-DDK_S172A_M173A_V195A RAD23A

    Plasmid

    #201576

    Purpose

    Expresses a variant of human RAD23A containing mutations S172A, M173A and V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutations S172A, M173A and V195A

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Stx5L(m55V)-GFP

    Plasmid

    #209845

    Purpose

    Expresses Stx5 M55V mutant fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    STX5 (STX5 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Methionine 55 to Valine, causes loss of S…

    Promoter

    CMV

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-6P-1-gp78 RING

    Plasmid

    #185353

    Purpose

    RING domain-containing GST fusion protein for ubiquitination assays.

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    gp78 cytoplasmic domain (AMFR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Stop codon after aa 393

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. B-HypaR-SpCas9

    Plasmid

    #126764

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Hypa2SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A; N692A; M694A; Q695A; H698A; amino acids 10…

    Promoter

    CBh

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ERAP1-rs27895(C)

    Plasmid

    #206142

    Purpose

    Expresses the rs27895-C allele of ERAP1, myc-tagged.

    Depositor

    Dennis Ko

    Article

    Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

    Insert

    ERAP1 (ERAP1 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-6P-1-gp78 429-611

    Plasmid

    #185354

    Purpose

    GST fusion protein for quantification of gp78 levels

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    gp78 (AMFR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 429-611 only

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-6P-1 AUP1 292-410 5E

    Plasmid

    #185332

    Purpose

    Bacterial expression of AUP1 with GST fusion protein for binding assays

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 292-410 only, with mutations R382E/Q383E/K390E…

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-6P-1 AUP1 292-410 A397D

    Plasmid

    #185333

    Purpose

    Bacterial expression of AUP1 with GST fusion protein for binding assays

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 292-410 only, with mutation A379D

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-C1-G2BRgp78

    Plasmid

    #185344

    Purpose

    Expression of soluble G2BR, amino acids 575-600 of human gp78

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    gp78 aa 574-600 (G2BR) (AMFR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 575-600 only

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pETDUET-GST-AUP1 379-410:Ube2G2

    Plasmid

    #185335

    Purpose

    Used for co-expression of the AUP1 G2BR with UBE2G2 for crystallography

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 aa 379-410, Ube2G2 (AUP1 Mouse, Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Cas9_ANKRD1_sgRNA2

    Plasmid

    #186669

    Purpose

    Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

    Plasmid

    #165083

    Purpose

    gRNA 2 of pair B for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

    Plasmid

    #165085

    Purpose

    gRNA 1 of pair C for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

    Plasmid

    #165086

    Purpose

    gRNA 2 of pair C for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTwist-SARS-CoV-2 Δ18 B.1.351v3/wtRBD

    Plasmid

    #169468

    Purpose

    Encodes SARS-CoV-2 B.1.351v3/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v3/wtRBD Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTwist-SARS-CoV-2 Δ18 B.1.351v1/wtRBD

    Plasmid

    #169466

    Purpose

    Encodes SARS-CoV-2 B.1.351v1/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v1/wtRBD Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSico_U6-PGC1a1 sgRNA

    Plasmid

    #165425

    Purpose

    Expression of gRNA against human PGC-1a variant 1

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    gRNA against PGC-1a variant 1 (PPARGC1A Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

29. pPGK-T7/2-CD44v2-10 (-cyt)

    Plasmid

    #137825

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v2-10 (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region - N213S on the CD44 re…

    Promoter

    PGK

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCri-H6-TEV-GRAB

    Plasmid

    #128498

    Purpose

    Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
    

    Use

    Tags

    His tag (6x)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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