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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,640 results for: dykddddk

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Showing: 4821 - 4840 of 8640 results

  2. pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

    Plasmid
    #135013
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    135013-plasmid-map-sequence-id-267601

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  7. pCAG-T3-st1CAS9-pA

    Plasmid
    #131542
    Purpose
    Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Publication
    Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized st1Cas9
    Use
    CRISPR
    Tags
    Flag, NLS, and polyA
    Expression
    Mammalian
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131542-plasmid-map-sequence-id-262465

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  8. pCAG-T3-SpCAS9-NG-pA

    Plasmid
    #131465
    Purpose
    Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Publication
    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized SpCas9-NG
    Use
    CRISPR
    Tags
    Flag, NLS, and polyA
    Expression
    Mammalian
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131465-plasmid-map-sequence-id-262447

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  12. pcDNA-LmoCas6-p300

    Plasmid
    #126486
    Purpose
    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LmoCas6-p300
    Use
    CRISPR
    Tags
    Flag and Flag, NLS
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126486-plasmid-map-sequence-id-246633

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Showing: 4821 - 4840 of 8640 results