We narrowed to 5,822 results for: pCas

1. pBSE901

   Plasmid

   #91709

   Purpose

   CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2T-CMV-eA3A-BE5-NG-BlastR

   Plasmid

   #152998

   Purpose

   High purity C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPK1520_puroR

   Plasmid

   #173901

   Purpose

   SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

   Depositor

   Ronald Cohn

   Article

   Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV/U6

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. YE2-BE4max

   Plasmid

   #138156

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(YE2)-nSpCas9-UGI-UGI
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 W90Y + R132E; within Cas9 D10A

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQAT25

   Plasmid

   #223397

   Purpose

   T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by AtUBQ10 and sgRNA was driven by AtU3; Hygromycin for plants select.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPDK3860

   Plasmid

   #199245

   Purpose

   Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Promoter

   pPEBV

   Available Since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TC1681

   Plasmid

   #164872

   Purpose

   CURE-C2: C to U RNA Editing, with high efficiency and high off-target effects

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   hAPOBEC3A fused to PspCas13b (APOBEC3A )
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   human apobec Y132D

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMTKM05

   Plasmid

   #233477

   Purpose

   SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Hyg selection.

   Depositor

   Vincent Martin

   Article

   Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. SQT1665

   Plasmid

   #78744

   Purpose

   Human expression plasmid for LbCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX458-ECFP

    Plasmid

    #112220

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    ECFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. SQT1659

    Plasmid

    #78743

    Purpose

    Human expression plasmid for AsCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. ePPEmax*

    Plasmid

    #205244

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    Detailed in manuscript

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Double UP mNeon to mScarlet Rac1-V12

    Plasmid

    #125136

    Purpose

    Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).

    Depositor

    Erik Dent

    Article

    Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

    Insert

    mNeon/mScarletP2A Rac1-V12
    

    Expression

    Mammalian

    Mutation

    Removed Not1 site in mScarlet. 12th AA in Rac1 ch…

    Promoter

    CAG

    Available Since

    May 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-PITCh-PXN

    Plasmid

    #227315

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PXN for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PXN (PXN Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG sHRPb-FKBP-post mGRASP

    Plasmid

    #73146

    Purpose

    Small fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPb-FKBP-post mGRASP
    

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. NG-ABE8-dimer

    Plasmid

    #138497

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9 (NG)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPomyces-1

    Plasmid

    #61736

    Purpose

    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    tracrRNA
    crRNA cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAG-Cremyc-2A-GFP

    Plasmid

    #116879

    Purpose

    Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

    Depositor

    Qiang Lu

    Article

    Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

    Insert

    Cremyc-2A-GFP
    

    Expression

    Mammalian

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. A3A-PBE

    Plasmid

    #119768

    Purpose

    Conversion of C to T in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A and E945K in wild type …

    Promoter

    maize Ubiquitin-1

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. GB4542

    Plasmid

    #215261

    Purpose

    Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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