We narrowed to 9,316 results for: Coli

1. pdCas9-M-3F2

   Plasmid

   #73428

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor C4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLY54

   Plasmid

   #130923

   Purpose

   A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dcas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   

   Use

   Synthetic Biology

   Tags

   ASV tag and pspFΔHTH::λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Anderson promoter: J23106, PpspA-LEA2B2, and Ptet

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. N229A (EcQ) MetNIQ in pBAD

   Plasmid

   #118257

   Purpose

   N229A Mutation in Methionine Binding Protein (MetQ) and Wild Type Methionine ABC Transporter for Transport Activity Assay

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetN (metN E. coli)
   MetI (metI E. coli)
   MetQ (N229A) (metQ E. coli)
   

   Use

   Tags

   10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 229 to Alanine (Ala) and…

   Promoter

   None and araBAD Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. N295A (EcN), N229A (EcQ) MetNIQ in pBAD

   Plasmid

   #118581

   Purpose

   N295A Mutation of MetN in Methionine ABC Transporter and N229A Mutation in Methionine Binding Protein (MetQ) for Transport Activity Assay

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetN (metN E. coli)
   MetI (metI E. coli)
   MetQ (metQ E. coli)
   

   Use

   Tags

   10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 229 to Alanine (Ala) and…

   Promoter

   None and araBAD Promotoer

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. N295A (EcN) MetNIQ in pBAD

   Plasmid

   #118256

   Purpose

   N295A Mutation of MetN in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetN (N295A) (metN E. coli)
   MetI (metI E. coli)
   MetQ (metQ E. coli)
   

   Use

   Tags

   10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 295 to Alanine (Ala)

   Promoter

   None and araBAD Promotoer

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. N295A (EcN), Y160A (EcI) MetNIQ in pBAD

   Plasmid

   #118260

   Purpose

   N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetN (N295A) (metN E. coli)
   MetI (Y160A) (metI E. coli)
   MetQ (metQ E. coli)
   

   Use

   Tags

   10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

   Expression

   Bacterial

   Mutation

   Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

   Promoter

   None and araBAD Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHE111-3xFlag-HSP70

   Plasmid

   #236055

   Purpose

   Mammalian expression of human HSP70 with a N-terminal 3x-FLAG tag

   Depositor

   Jonathan Schisler

   Article

   Stewart et al bioRxiv 2025.02.17.637997

   Insert

   HSP70 (HSPA1A Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Aug. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAD100-CHIP-myc-His

   Plasmid

   #236054

   Purpose

   Mammalian expression of human CHIP with a C-terminal myc-His tag

   Depositor

   Jonathan Schisler

   Article

   Stewart et al bioRxiv 2025.02.17.637997

   Insert

   CHIP (STUB1 Human)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Aug. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAD120-CHIP-G132N-myc-His

   Plasmid

   #236053

   Purpose

   Mammalian expression of human CHIP with the synthetic G132N substitution and C-terminal myc-His tag

   Depositor

   Jonathan Schisler

   Article

   Stewart et al bioRxiv 2025.02.17.637997

   Insert

   CHIP-G132N (STUB1 Human)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   G132N

   Promoter

   CMV

   Available since

   Aug. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAD000-CHIP-K30A-myc-His

    Plasmid

    #236052

    Purpose

    Mammalian expression of human CHIP with the synthetic K30A substitution and C-terminal myc-His tag

    Depositor

    Jonathan Schisler

    Article

    Stewart et al bioRxiv 2025.02.17.637997

    Insert

    CHIP-K30A (STUB1 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    K30A

    Promoter

    CMV

    Available since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pet28b-His_RAD23A-UBA2

    Plasmid

    #201550

    Purpose

    Expresses UBA2 of human RAD23A in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Expresses amino acids 311-363 of DNA polymerase i…

    Promoter

    T7

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJRM919_WT aa 1-419 His POLI

    Plasmid

    #201445

    Purpose

    Low copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLI

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Expresses amino acids 1-449 of DNA polymerase iot…

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLY315

    Plasmid

    #184160

    Purpose

    Reporter circuit for hijacking mRNA of zntA as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-zntA-1034, PrhaB, and Ptet

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLY300

    Plasmid

    #184158

    Purpose

    Reporter circuit for hijacking small RNA oxyS as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-oxyS-86, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLY320

    Plasmid

    #184161

    Purpose

    Reporter circuit for hijacking mRNA of cusCFAB as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-cusCFAB-656, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBGE2.0

    Plasmid

    #165987

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Tet resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    tetracycline resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psgRNA-2xMS2-lacO

    Plasmid

    #181909

    Purpose

    Single guide RNA with 2XMS2 loops targeting bacterial lac operator

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    sgRNA-2XMS2-lacO
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLY9

    Plasmid

    #130907

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-2G6, and Ptet

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLY59

    Plasmid

    #130927

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    sfgfp
    pspFΔHTH::MCP
    

    Use

    Synthetic Biology

    Tags

    ASV tag and MCP (MS2 coat protein)

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-2G6, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLY57

    Plasmid

    #130926

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA5B5, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge