We narrowed to 12,952 results for: sequence
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Plasmid#207541PurposeHomologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human XRCC4 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
N-terminal Halo-Ku70
Plasmid#207547PurposeHomologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus.DepositorInsertHaloTag with flanked by human Ku70 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWR58
Plasmid#202795Purposeintegrating bioluminescene reporter for Cas9 activity in S. stipitisDepositorInsertsP-TDH3/coCBG(first portion)-YUM1
P-TEF1/ShBle/T-ACT1
YUM1-coCBG(last portion)/T-ADH2
URA3 and flanking sequence
UseTagsExpressionYeastMutationPromoterAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh13CDS
Plasmid#192853PurposePlasmid to produce ISH probe for mMyh13 gene with CDS sequenceDepositorAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh13UTR
Plasmid#192854PurposePlasmid to produce ISH probe for mMyh13 gene with UTR sequenceDepositorAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAMTr2GCsgSeLEU2
Plasmid#224870PurposeDisruption of S. eubayanus type LEU2 genesDepositorInsertpYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAMTrGCsgScLEU2
Plasmid#224869PurposeDisruption of S. cerevisiae type LEU2 genesDepositorInsertpYAMTrGCas having the guide sequence ScLEU2 (LEU2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAMTr2GCsgScLEU2
Plasmid#224868PurposeDisruption of S. cerevisiae type LEU2 geneDepositorInsertpYAMTr2GC having the guide sequence ScLEU2 (LEU2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP-EV
Plasmid#209973PurposeContains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmidsDepositorInsertCDS (mock)
UseTagsExpressionBacterialMutationPromoterAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-NIR-FbLAG30
Plasmid#220748PurposeNIR-FbLAG30 expression in bacteria.DepositorInsertNIR-FbLAG30
UseAffinity Reagent/ AntibodyTagsExpressionBacterialMutationFor bacterial cytoplasmic expression, the pelB si…PromoterT7Available SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2(intΔNACs):GFP
Plasmid#218570PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
UseTagsExpressionPlantMutationMutation genomic sequence 85nt, 86nt from TA to C…PromoterAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_zCas9
Plasmid#213913PurposeEntry clone containing 3×FLAG-NLS-zCas9-NLS encoding sequence, Pisum sativum rbcS E9 terminator. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertzCas9
UseCRISPRTagsExpressionMutationZea mays codon-optimized Cas9PromoterAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
TR knockout HRD
Plasmid#207579PurposeHomologous recombination donor replacing TR with a puro cassette.DepositorInsertpuro resistance cassette flanked by TR locus sequences excluding TR
UseTagsNoneExpressionMammalianMutationPromoterSV40Available SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGC-LexACasPea
Plasmid#210539PurposeCloning estradiol-inducible expression cassette containi LexA XVE binding sequence followed by SpCas9 CDS and PEA3AT as GreenGate module CDepositorInsertLexA::SpCas9::PEA3AT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD043
Plasmid#212913PurposeEncodes the S. cerevisiae 111 amino acid SCW4 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD041
Plasmid#212911PurposeEncodes the S. cerevisiae YAR066W Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD042
Plasmid#212912PurposeEncodes the S. cerevisiae HSP150 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD044
Plasmid#212914PurposeEncodes the S. cerevisiae 142 amino acid SCW4 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP-sfgfp
Plasmid#209994PurposeContains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmidsDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 genomic A. thaliana PLDGAMMA3
Plasmid#209188PurposepENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tagsDepositorInsertAT4G11840 (PLDGAMMA3 Mustard Weed)
UseGateway-compatible entry vectorTagsExpressionMutationPromoternoneAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB395
Plasmid#203631PurposeTU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).DepositorInsertR1:G1aG2b.1:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB396
Plasmid#203632PurposeTU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).DepositorInsertR1:G1ab.1:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB397
Plasmid#203633PurposeTU for the expression of luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).DepositorInsertR1:G1abc.3:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF_641-1574_AH
Plasmid#148936PurposeInsect Expression of DmGIGYF1_641-1574DepositorInsertDmGIGYF1_641-1574 (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-F361AF367A_AH
Plasmid#148937PurposeInsect Expression of DmGIGYF-F361AF367ADepositorInsertDmGIGYF-F361AF367A (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-W349A_AH
Plasmid#148938PurposeInsect Expression of DmGIGYF-W349ADepositorInsertDmGIGYF-W349A (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-WFFtoAAA_AH
Plasmid#148939PurposeInsect Expression of DmGIGYF-WFFtoAAADepositorInsertDmGIGYF-WFFtoAAA (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-YYMYFWFtoAAAAAAA_AH
Plasmid#148941PurposeInsect Expression of DmGIGYF1-YYMFWFtoAAAAAAADepositorInsertDmGIGYF1-YYMFWFtoAAAAAAA (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-PPFPPFtoAAAAAA_AH
Plasmid#148908PurposeInsect Expression of DmHPat-PPFPPFtoAAAAAADepositorInsertDmHPat-PPFPPFtoAAAAAA (Patr-1 Fly)
UseTagsExpressionInsectMutationthree silent mutations compared to the sequence g…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4EHP-W114A_AH
Plasmid#148909PurposeInsect Expression of Dm4EHP-W114ADepositorInsertDm4EHP-W114A (4EHP Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only